from Sueoka, N. (1960) Proc. Natl. Acad. Sci. USA 46, 83-91.
Also known as HS or HSM, this is somewhat more economical than TAP to prepare.
Make the following stock solutions:
1. salts solution (also known as Beijerinck's solution)
NH4Cl | 100.0 g | |
MgSO4 . 7H2O | 4.0 g | |
CaCl2 . 2H2O | 2.0 g | |
water to 1 liter |
2. phosphate solution
K2HPO4 | 288.0 g | |
KH2PO4 | 144.0 g | |
water to 1 liter |
3. Hutner's trace elements (follow this link)
To make the final medium, mix the following:
5 ml solution #1 (salts)
5 solution #2 (phosphate)
1.0 ml solution #3 (trace elements)
water to 1 liter
Sodium acetate can be added at 1.2 g/liter (anhydrous) or 2.0 g/liter (hydrate).
For solid medium, add 15 g agar per liter.
Yeast extract 4.0 g/liter can also be added to solid medium.
All these ingredients can be added before autoclaving.
We don't recommend adding yeast extract to liquid media, since it encourages overgrowth of contaminating bacteria.
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