pMJ504: pLM005-Cre12.g498550-Venus-3xFLAG

MPM1 (Cre12.g498550) gDNA coding sequence, spanning the start codon to the triplet just before the stop codon, was cloned in frame into pLM005 by Gibson assembly (as described in https://doi.org/10.1073/pnas.1522866113.

Selection in E. coli: ampicillin
Selection in C. reinhardtii: paromomycin
Host strain: DH5α
Transformed into C. reinhardtii strain cMJ030 to produce CC-5649

Plasmid: 1..9696 bp DNA circular
CDS	0784..1587	“APHVIII"
CDS	2699..5694	“MPM1”
CDS	5695..6519	“VENUS-3xFLAG fusion protein”
TRANSLATION: NGDLGGSGGRMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELIKGGGDYKDHDGDYKDHDIDYKDDDDK-

Plasmid sequence	1..9696
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGATATCCTTGACATGATTGGTGCGTATGTTTGTATGAAGCTACAGGACTGATTTGGCGGGCTATGAGGGCGGGGGAAGCTCTGGAAGGGCCGCGATGGGGCGCGCGGCGTCCAGAAGGCGCCATACGGCCCGCTGGCGGCACCCATCCGGTATAAAAGCCCGCGACCCCGAACGGTGACCTCCACTTTCAGCGACAAACGAGCACTTATACATACGCGACTATTCTGCCGCTATACATAACCACTCAGCTAGCTTAAGATCCCATCAAGCTTGCATGCCGGGCGCGCCAGAAGGAGCGCAGCCAAACCAGGATGATGTTTGATGGGGTATTTGAGCACTTGCAACCCTTATCCGGAAGCCCCCTGGCCCACAAAGGCTAGGCGCCAATGCAAGCAGTTCGCATGCAGCCCCTGGAGCGGTGCCCTCCTGATAAACCGGCCAGGGGGCCTATGTTCTTTACTTTTTTACAAGAGAAGTCACTCAACATCTTAAAATGGCCAGGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGTGTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGGATGGCCACTCCGCCCTCCCCGGTGCTGAAGAATTTCGAAGCATGGACGATGCGTTGCGTGCACTGCGGGGTCGGTATCCCGGTTGTGAGTGGGTTGTTGTGGAGGATGGGGCCTCGGGGGCTGGTGTTTATCGGCTTCGGGGTGGTGGGCGGGAGTTGTTTGTCAAGGTGGCAGCTCTGGGGGCCGGGGTGGGCTTGTTGGGTGAGGCTGAGCGGCTGGTGTGGTTGGCGGAGGTGGGGATTCCCGTACCTCGTGTTGTGGAGGGTGGTGGGGACGAGAGGGTCGCCTGGTTGGTCACCGAAGCGGTTCCGGGGCGTCCGGCCAGTGCGCGGTGGCCGCGGGAGCAGCGGCTGGACGTGGCGGTGGCGCTCGCGGGGCTCGCTCGTTCGCTGCACGCGCTGGACTGGGAGCGGTGTCCGTTCGATCGCAGTCTCGCGGTGACGGTGCCGCAGGCGGCCCGTGCTGTCGCTGAAGGGAGCGTCGACTTGGAGGATCTGGACGAGGAGCGGAAGGGGTGGTCGGGGGAGCGGCTTCTCGCCGAGCTGGAGCGGACTCGGCCTGCGGACGAGGATCTGGCGGTTTGCCACGGTGACCTGTGCCCGGACAACGTGCTGCTCGACCCTCGTACCTGCGAGGTGACCGGGCTGATCGACGTGGGGCGGGTCGGCCGTGCGGACCGGCACTCCGATCTCGCGCTGGTGCTGCGCGAGCTGGCCCACGAGGAGGACCCGTGGTTCGGGCCGGAGTGTTCCGCGGCGTTCCTGCGGGAGTACGGGCGCGGGTGGGATGGGGCGGTATCGGAGGAAAAGCTGGCGTTTTACCGGCTGTTGGACGAGTTCTTCTGAGGGACCTGATGGTGTTGGTGGCTGGGTAGGGTTGCGTCGCGTGGGTGACAGCACAGTGTGGACGTTGGGATCCCCGCTCCGTGTAAATGGAGGCGCTCGTTGATCTGAGCCTTGCCCCCTGACGAACGGCGGTGGATGGAAGATACTGCTCTCAAGTGCTGAAGCGGTAGCTTAGCTCCCCGTTTCGTGCTGATCAGTCTTTTTCAACACGTAAAAAGCGGAGGAGTTTTGCAATTTTGTTGGTTGTAACGATCCTCCGTTGATTTTGGCCTCTTTCTCCATGGGCGGGCTGGGCGTATTTGAAGCGGGTACCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGACCAATCGTCACACGAGCCCTCGTCAGAAACACGTCTCCGCCACGCTCTCCCTCTCACGGCCGACCCCGCAGCCCTTTTGCCCTTTCCTAGGCCACCGACAGGACCCAGGCGCTCTCAGCATGCCTCAACAACCCGTACTCGTGCCAGCGGTGCCCTTGTGCTGGTGATCGCTTGGAAGCGCATGCGAAGACGAAGGGGCGGAGCAGGCGGCCTGGCTGTTCGAAGGGCTCGCCGCCAGTTCGGGTGCCTTTCTCCACGCGCGCCTCCACACCTACCGATGCGTGAAGGCAGGCAAATGCTCATGTTTGCCCGAACTCGGTGTCCTTAAAAAGCCGCTTCTTGTCGTCGTTCCGAGACATGTTAGCAGATCGCAGTGCCACCTTTCCTGACGCGCTCGGCCCCATATTCGGACGCAATTGTCATTTGTAGCACAATTGGAGCAAATCTGGCGAGGCAGTAGGCTTTTAAGTTGCAAGGCGAGAGAGCAAAGTGGGACGCGGCGTGATTATTGGTATTTACGCGACGGCCCGGCGCGTTAGCGGCCCTTCCCCCAGGCCAGGGACGATTATGTATCAATATTGTTGCGTTCGGGCACTCGTGCGAGGGCTCCTGCGGGCTGGGGAGGGGGATCTGGGAATTGGAGGTACGACCGAGATGGCTTGCTCGGGGGGAGGTTTCCTCGCCGAGCAAGCCAGGGTTAGGTGTTGCGCTCTTGACACGTTGTGCATTCTAGGACCCCACTGCTACTCACAACAAGCCCAGTTATGCAGTGCCTAAACACCAAGCCTTTTACCTCGGCCCGCCCGGCTGTGGCCCGGCGGAGCGTCGTCGCGCATGCCTCGCGCCCGGCCCAGGCTGCCGCGCTGGCCTCTGCCATGACCGTGGCGACCACCTCCGCTGCTATGGCTTCTGAGATTGCTCAAACTGCCGATGTTGGCTCGCTGACCTTCGCGGTTGGCGGAGTGGGCGCTGTTGTGGGCCTGGGCGCCCTGCTTGTCGCTACTGACCCTCAGAAGCGGTGAGAAGGGTGCAGGAGTGCCTCAGATCTTAATGCAGATGCGCGTGCTTGGCTAGGATGGCATCACGCAAGCAAACATCGCGTTTCTGCTCCTCGCAGCCGCTCGGAGCAGATGAAGTCATTCGACGGCGATGAGAAGGAGGCGGTGAGTCCAAAATACAGGCTTCAATTTGAGTTGGAGGCAGCAATCCGACCCTGCGCCCCAGCTGTGTTCTGCGCCGGGTATGGGGACGTGCCACCCCTCTCCGCGGATCCGGTCTTCTCACGGACTGGGTTTGCTGTGAATTGTCCGGACAGCCACCGACGAAGTTCAGCATCATCTACCGCTACCCGGATACCCTGGCAGATCTAGTGTTGCAGCTTGATATCAGCAGCTAGCGCGACAACCACGCCAGCAACAGCCACGTCGCTCCGCGCACTCGCGCCCGCAGCGACCACCTCCCCTCACCCCCTCCCCCCATCACCCCATCAGGTGAAGGACTACTTCAACACTGCCGGCTTCGAGCGCTGGCGCAAGATCTACGGCGAGACGGACGAGGTGAACAAGGTGCAGCTGGACATCCGCACTGGCCACGCGCAGACGGTGGACAAGGTGCTGCGCTGGGTGGACGAGGAGGGCAGCGTGCAGGGCATCACCGTGGCGGACTGCGGCTGCGGGACAGGTGGGAGGGGCAGAGGGCCGTGGGCCGGGGGGTAGAAGGAGGGGCAGGAGGAGGGGAGGAGGCTGTCGCGGGGATGGCGCTGGTTGGCGTGCGGGTAGAGGGTAAGAGGGCGGAAGGGGCTGGGGCAGGTGAACGTGTTTGGAAAGGTTGCAGGAAGCACGGACATGGTGCACGGACAGAAGGCTTATGATGACGCAATAGCAGGGCGTGCCGAGTCGTGGGCGTACCCCGCGGCCAAGAATGCAATTCGGCAGCGCCAAGCAGTTTGCTTGGCAAGCCAGCACTATGGTATTCCCACCGCCGGAGGCGACGACCCACACATGCTTAGCACGACCTCTCCCGGCAACTCATGACCGCCTCTCCTCCCGCTCCCTCTGCCCCTCGGCACACCTCCTCCCAACACACCACCCACAGGCAGCCTGGCCATCCCGCTGGCGCTGCGCGGCGCCGCCGTCAGCGCCTCCGACATCTCCGCCGCCATGGCGTCTGAGGCGGAGCAGCGCTACCAGCAGGCGGTGGCGGCCGGGCAGGGCAAGGCGCCCAAGGTGGCGCCCAAGTTCGAGGCGCTGGACCTGGAGTGAGTGCTGGGGAGAGAGAGGACGGGAGAGGGAGGGCGCGAGGAAGGAACGAGGGAACAAAGGGGAGGGGGATAGGAGAACTGGTGGTTGGAACAAAAGCACGCGAACACAATGGAGGCGGGAAGCAGATCAGGCGGGGAAATATGAAAGCGGAGGAGGGTAGGAGGGCCGAGGTCAGGGGAGGAAGGAAAAGGGCCGCAAGACGGGAGGCGCTGTCGGGCATGGAAGCTGAATGGAGGACGGCAAGAAGTGTCTGCGGGCGCGCCGTGAATGGACACGGTGGTGGTGGTGGGGGGGGGGGGTAGTTCATTCCACTTCCATTATTAACCACTGCCAATCGGGGAAGAGTGCGAGGGCGGAAAGGGATGAGGGCCAGCCAATGGCTCCAGCGCTGTTGTTCACGAGGCGACCAGCGCGGCTGGAGGGGACCATCATCACATCATGGCGAGCGCAGGGCGTAGGACTCCGTGGAACGGAACGGGACGGGAAAAGGGACGGGGTCGGAAGTCGGGTTGGAGGGGGCCCGCGACCGAGCGCGGGCGAGGGCTGCCGTGCGTGCGTGTTTCCGAACCCCAACCGACCAAGCTGTCGCCTTTCCGCACCCGCCCTCACTGCCGCAGGTCCGTCAAGGGCAAGTACGACACGGTCACTTGCCTGGACGTGATGATTCACTACCCCCAGGTGCGGCCGGGGCCCGGTGCGGATTGGGCGGGCGTGTCGTTTGCACACGTTGTGTGTTGGAAAGAGTCAACTGCTAGCCCACATGAACCCATACAAGCCGTGGGGAAATGTGGCGAGTGCAATTGTATGTCACATATGCCATTTGGGGCGGGGAGGGCGGGGCTGCTGACAGTGGTGGACTGGGCTGCAGCGTTTGGCGGTGACTGAAATGGCACACCGGTGCAGGCCCCTGTGCCACAGCCGCGTGCGCTTTGAAGCTCGGGGACAGCCCTCACAGCAGCATCGTCTGGCCGCGCTCCGCCTGCTTACGCCGTTTCCTCTCTCCCGCGCCCTCCTCACCCCGCAGGACAAGGTGGACGCCATGATCACGCACCTGGCGGGTCTGTCCGACCGCCGCCTCATCATCTCGTTCGCACCCAAGACGCTGTCGTACAGCATCCTCAAGCGCATTGGCGAGCTGTTCCCCGGCCCCAGCAAGGTGCGCAGCCTGCGAAGGGGCAGGGCTGATGTGCACTTATTGGGGTGCAATGACGAGCTGACGTTGTTGTTCCAAGTGGTTGCTACCGGCGGTGCATGGCACTGGGATGTGGGGGCACACGGCGCCTTGAGGTGTCGCCGATGCTACCGCATGTCGCAGCGTTGTTACACCGTGTGGCCCCTCGTCCCACCCATGGCCCGCCTTCGTGCAGGCCACCCGCGCCTACCTGCACCGTGAGGAGGACGTGGAGGCTGCCCTCAAGCGCGCCGGCTTCAAGGTCACCAAGCGTGAGATGACCGCCACCAGCTTCTACTTCTCGCGCCTGCTGGAGGCCATCCGCGAGAACGGAGATCTGGGTGGCTCCGGCGGCCGGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGACGGCGACGTGAACGGCCACAAGTTCAGCGTGAGCGGCGAGGGCGAGGGCGACGCCACCTACGGCAAGCTGACCCTGAAGCTGATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGCCACAAGCTGGAGTACAACTACAACAGCCACAACGTGTACATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTGGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGAGCGCCCTGAGCAAGGACCCCAACGAGAAGCGCGACCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGACGAGCTGATCAAGGGCGGTGGCGACTACAAGGACCATGACGGTGACTATAAGGATCACGACATCGACTACAAGGACGATGACGACAAGTAAGATCTCTAACTGCACGAATTCTCAGGTCGGGACATCCCCGACCAGGGGCGGCGGGGATGTTGCTGGGCCGATGGAAAGTAGCAACCCAGCCAGCGGCTTCCAGCGCACTCCAGCTGCTCACGGTTGCGACATTGCGCGTGCACGCTTGCGCGTCCCTCACTCGGCCAGCTTGTCGCCGCAGACATCCCTAGCATTGTGCGGACTGCGGTCGTCAGTTAGCGTAGTGGCGGGGCTCAAAGCGTGATGCAGCTGGTGGCTGATTGCATGTGCTACATATGCTGTTATGTTTTGCATGAACTTCGATGCATTGGATGCTGGGTGCACGCGTTTGCATGTGTTTGTGCCGGCATGCTGCCGTCGTCGGCCGTACGTTTACGTTTCTGTGTGCCGGGGTCTTTATTTCCGCCTGCAGACCGACGTCGGTTCCACTAGTTCTAGATGGCAGCAGCTGGACCGCCTGTACCATGGAGAAGAGCTTTACTTGCCGGGATGGCCGATTTCGCTGATTGATACGGGATCGGAGCTCGGAGGCTTTCGCGCTAGGGGCTAGGCGAAGGGCAGTGGTGACCAGGGTCGGTGTGGGGTCGGCCCACGGTCAATTAGCCACAGGAGGATCAGGGGGAGGTAGGCACGTCGACTTGGTTTGCGACCCCGCAGTTTTGGCGGACGTGCTGTTGTAGATGTTAGCGTGTGCGTGAGCCAGTGGCCAACGTGCCACACCCATTGAGAAGACCAACCAACTTACTGGCAATATCTGCCAATGCCATACTGCATGTAATGGCCAGGCCATGTGAGAGTTTGCCGTGCCTGCGCGCGCCCCGGGGGCGAAGCTTGGTACCGCTAGCGTCAGCTGAACTCCTGCTGCCACGCTGACAGAGACGCGCCCATGACGTAGCGCCGACTGCTGCTCCTCTGCTCGTGGAGCTCTGAATCTGCTGCCGGCTGCCCCCGCCCCCGCTGCTGAGGCTGAATCTACTCCGCTCTCCACCCCAGTCCCAGGAAGAGAGGCGCATTTACTTCGCACAGACGTTACAGCACACCCTTGATCATCATCAGCTGCTCTTCCCTGCCGCTGCAACACGCCCCGCGCGATATCCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC