HS, HSA and YA

HS (Sueoka’s high salt medium), HSA and YA recipes 

from Sueoka, N. (1960) Proc. Natl. Acad. Sci. USA 46, 83-91.

 

HS media (no acetate)

5 ml Beijerinck’s solution

5 ml of phosphate solution

1 ml of Hutner’s trace elements

water to 1 L

 

HSA media (with acetate)

5 ml Beijerinck’s solution

5 ml of phosphate solution

1 ml of Hutner’s trace elements

1.2 grams sodium acetate (anhydrous); for the hydrated salt use 2.0 g

water to 1 L

 

YA media (with acetate and yeast extract)

5 ml Beijerinck’s solution

5 ml of phosphate solution

1 ml of Hutner’s trace elements

1.2 grams sodium acetate (anhydrous); for the hydrated salt use 2.0 g

4 grams yeast extract

water to 1 L

 

 

Stock Solutions

1)    Beijerinck’s solution  (Complete salts solution)

NH4Cl                                    100 g

MgSO4 ∙ 7H2O                     4.0 g

CaCl2 ∙ 2H2O                        2.0 g

water to 1 liter

For sulfur-free salts solution, substitute 3.30 g MgCl2 ∙ 6H2O for the magnesium sulfate.
For N- medium for gametogenesis, omit the ammonium chloride.

 

2)    Phosphate solution

K2HPO4 (anhydrous)            288.0 g

KH2PO4                                   144.0 g

water to 1 liter

 

3)    Hutner’s Trace Elements

 

Keep the stock solutions at 4°C
For plates or slants, use 12 grams agar per liter

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