These are sent as phage in suspension. Instructions for working with the libraries can be obtained from the Agilent web site (formerly Stratagene). Enter catalog #236201 in the search box on this page. We recommend the Agilent host strain XL1 Blue MRF’ for these libraries.

The libraries are available to academic and other non-profit users for $250 each, and to commercial institutions for $500 each.

Generation and Organization of Chlamydomonas cDNA Information

Core

$250.00

C. reinhardtii CC-1690, Lambda Zap II

Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from CC-1690 cells grown to mid-log phase in TAP (acetate-containing) medium in the light, TAP medium in the dark, HS (minimal) medium in ambient levels of CO2 and HS medium bubbled with 5% CO2.

ESTs from this library are identified with the project IDs 874, 894 and 1024.


Stress I

$250.00

C. reinhardtii CC-1690, Lambda Zap II

Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from CC-1690 cells grown to mid-log phase in TAP-N (30 min, 1hr, 4hr), TAP-S (30 min, 1hr, 4hr), TAP-P (4hr, 12hr, 24hr), NO3 to NH4 (30min, 1hr, 4hr) and NH4 to NO3 (30min, 1hr, 4hr).

ESTs from this library are identified with the project ID 963.


Stress II

$250.00

C. reinhardtii CC-1690, Lambda Zap II

Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from CC-1690 cells grown to mid-log phase in TAP (NH4+ – containing) and shifted to TAP – NO3- (24hrs); H2 production conditions (0, 12hr, 24hr) see Melis et al.,(2000) Plant Phys. 122: 127-135; TAP + H2O2 (1, 12, 24 hr); TAP + sorbitol (1, 2, 6, 24 hr); TAP + Cd (1, 2, 6, 24 hr).

ESTs from this library are identified with the project ID 1031.


Stress III

$250.00

C. reinhardtii CC-1690, Lambda Zap II

Library prepared by Jeanette Quinn and Chiung-Wen Chang. This library combines cDNAs from CC-1690 cells grown to mid-log phase in copper-free TAP medium (see Quinn and Merchant(1998) Methods in Enzymology, 2997:263-279)in a shaking(250 rpm) illuminated (about 100 micromole/m2/sec) incubator at 22 C (see Quinn and Merchant(1998) Methods in Enzymology, 2997:263-279); CC-1690 cells grown to mid-log phase in low Fe (1 micromolar Fe) TAP medium (see La Fontaine S, Quinn JM, Nakamoto SS, Page MD, Gohre V, Moseley JL, Kropat J, Merchant S.LaFontaine et al. (2002) Eukaryotic Cell, 1:736-757) in a shaking illuminated incubator (same conditions as above). CC-1690 cells were grown to mid-log phase in TAP medium in a shaking illuminated incubator to a density of 8x10e6 cells/ml. The flask was transferred to a shaking platform (200 rpm) at room temperature (23C) 12 micromole/m2/sec illumination and bubbled in a stoppered flask with 98% nitrogen, 2% CO2 gas mixture for 1 hour prior to harvesting for RNA isolation (as per Quinn JM, Barraco P, Eriksson M, Merchant S. Quinn et al. (2000) JBC 275:6080-6089); CC-1690 cells grown to mid-log phase (3x10e6 cells/ml) in TAP medium in a shaking (150 rpm) illuminated (70 mole photon/m2/sec) incubator at 27 C. Cells were diluted to 1x10e6 cells/ml, transferred to high light (11000 mol photon/m2/s) with shaking (150 rpm) and sampled at (0.5, 1,2,4,6, 12 hrs); CC-1690 cells grown to mid-log phase in HS medium in a shaking (150 rpm) illuminated (70 mole photon/m2/sec) incubator at 27 C. Cells were diluted to 1x10e6 cells/ml, transferred to high light (11000 mol photon/m2/s) with shaking (150 rpm) and sampled at (0.5, 1,2 ,4,6, 12 hrs). PolyA mRNA was purified from each sample, pooled and cDNA synthesized (see Shrager et al, Plant Physiol. 131, 401-408 for details). The cDNA was directionally cloned into lambda Zap II (Stratagene) in the EcoRI (5′) and XhoRI (3′) sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage. The library was normalized using method 4 described in Bonaldo et al., (1996) Genome Research 6: 791-806.

ESTs from this library are identified with the project ID 1115.


S1D2

$250.00

C. reinhardtii CC-2290, Lambda Zap II

Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from strain CC-2290 (Minnesota isolate of C. reinhardtii) grown to mid-log phase in TAP (acetate containing) medium in the light.

ESTs from this library are identified with the project ID 925.


Deflagellation

$250.00

Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from CC-1690 cells which had been re-synthesizing flagella for 15, 30 and 60 min after being deflagellated by pH shock. PolyA mRNA was purified from each sample, pooled and cDNA synthesized.

ESTs from this library are identified with the project ID 1030.


Gamete and Zygote

$250.00

Library prepared by Hui Zhao, Min Lu, Jeffrey McDermott, William J. Snell and John Davies. cDNA was prepared in the laboratory of William Snell from strain 21gr mt+ and from a mt- strain (Sager 6145, CC-1691) which had been transfered to nitrogen-deficient medium and sampled at 3, 8, 10, 12, 15, and 17 hours during the period of gametogenesis and at 30 and 60 minutes following mixing of the two mating types. PolyA mRNA was purified from each sample, pooled and cDNA synthesized.

ESTs from this library are identified with the project ID 1112.


-S

$250.00

-Sulfur


+-S/-Fe

$250.00

+-S/-Fe