From Jean-David Rochaix to Boynton-Gillham laboratory, 1981

Chloroplast DNA BamH1 14/15 (3.0 kb) from Rochaix’s cw15 strain in pBR313; contains 3′ end of 23S rRNA gene through 5S; see P-124 for same insert in pBR322

host strain: C600
amp resistant

 

More information

Strain: Rochaix cw15

Insert: chloroplast DNA BamHI 14/15 (3.0 kb)

Genes present: 23S rRNA (3′ end), 5S rRNA

Vector: pBR313

Bacterial host strain: C600

Selectable marker: amp-r tet-s

Origin: Single cell clone of P-3 (Ba1 from Rochaix received 6/81)

P-14

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

 

From Jean-David Rochaix to Boynton-Gillham laboratory, 1981

Chloroplast DNA BamHI 10 (7.6 kb) from Rochaix’s cw15 strain in pBR313; contains entire atpB gene and 3′ portion of ORF1995

host strain: C600
amp resistant

 

More information

Strain: Rochaix cw15

Insert: chloroplast DNA BamHI 10 (7.6 kb)

Genes present: atpB

Vector: pBR313

Bacterial host strain: C600

Selectable marker: amp-r tet-s

Origin: Single cell clone of P-6 (Ba 5 from Rochaix, received 6/81)

Reference: Boynton et al. (1988). See for use of this plasmid in transformation of atpB gene, and analysis of 12 bp tandem repeats./p>

P-17

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

From Jean-David Rochaix to Boynton-Gillham laboratory, 1981

Chloroplast DNA BamHI 8 (9.6 kb) from Rochaix’s cw15 strain in pBR313; contains most of rpoC2 (3′) and 5′ end of psaB; see P-122 for same insert in pBR322

host strain: C600
amp resistant

 

Additional Information

Strain: Rochaix cw15

Insert: chloroplast DNA BamHI 8 (9.6 kb)

Genes present: rpoC2 (3′ end), psaB (extreme 5′ end), ARS04,02

Vector: pBR313

Bacterial host strain: C600

Selectable marker: amp-r tet-s

Origin: Single cell clone of P-8 (Ba7 from Rochaix, received 6/81)

P-19

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

From Jean-David Rochaix to Boynton-Gillham laboratory, 1981

Chloroplast DNA BamHI 6 (13.4 kb) from Rochaix’s cw15 strain in pBR313; contains 3′ end of rps2 rpoA, psbB, psbN, and psbH; P-202 is the same insert in pBR322

host strain: C600
amp resistant

 

More information

Strain: Rochaix cw15

Insert: chloroplast DNA BamHI 6 (13.4 kb)

Genes present: psbH, psbN, ORF31, psbB, probably rps2

Vector: pBR313

Bacterial host strain: C600

Selectable marker: amp-r tet-s

Origin: Single cell clone of P-10 (Ba10 from Jean-David Rochaix, received 6/81

P-21

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA BamHI 9 (8.4 kb) in pUC8, from CC-125 wild type; contains rpl14, rps8, psaA exon 1, and rps4

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA BamHI 9 (8.4 kb)

Genes present: rpl14, rpl5, rps8, psaA (exon 1), rps4

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-57

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA BamHI 11/12 (7.0 kb) in pUC8, from CC-125 wild type; contains 16S rRNA through 23S 5′ end

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA BamHI 11/12 (7.0 kb)

Genes present: 16S rRNA, 23s rRNA (5′ end)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-59

See P-271 for the same insert in pBR322.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 5 (10.5 kb) in pUC8, from CC-125 wild type; contains psbB, rpoA, rps2 and most of rps18

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 5 (10.5 kb)

Genes present: psbB (5′), rps2, atpF

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Jeff Palmer in Boynton/Gillham lab, 1986

P-62

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 8 (8.5 kb) in pUC8, from CC-125 wild type; contains rps8, psaA exon 1, rps4 and 5′ end of 16S rRNA

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 8 (8.5 kb)

Genes present: rps8, psaA (exon 1), rps4, 16S rRNA (5′ end)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-63

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 9 (7.6 kb) in pUC8, from CC-125 wild type; contains clpP, rpl35, rpl23, and 5′ half of rpl2

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 9 (7.6 kb)

Genes present: clpP, trnL, petB, chlL, rpl23, rpl2 (5′)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-64

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 12 (6.5 kb) in pUC8, from CC-125 wild type; contains psaA exon 3

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 12 (6.5 kb)
Genes present: psaA (exon 3)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-65

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 15 (6.0 kb) in pUC8, from CC-125 wild type; contains psbA exons 1-4

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 15 (5.8 kb)psbA

Genes present: psbA exons 1-3

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-66

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 14 (5.8 kb) in pUC8, from CC-125 wild type; contains psaB, rbcL, and 5′ end of atpA

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 14 (5.5 kb)

Genes present: psaB, rbcL, atpA (5′)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-67

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 15 (5.8 kb) in pUC8, from CC-125 wild type; contains psbA exons 1-4

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 15 (5.8 kb) Gene psbA

Genes present: psbA (exons 1, 2, 3)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

Comment Same insert as P-66, but opposite orientation.

P-68

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 16 (5.0 kb) in pUC8, from CC-125 wild type; contains 3′ end of rpl2, rps19, rpl16, rpl14, and rpl5

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 16 (5.0 kb)

Genes present: rpl2 (part), rps19, rpl16, oriA, rpl14, rpl5

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-69

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

 

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 17 (4.9 kb) in pUC8, from CC-125 wild type; contains rps12, psaJ, atpI, and psbJ

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 17 (4.9 kb)

Genes present: psaJ, rps12

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-70

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 18 (4.9 kb) in pUC8, from CC-125 wild type; contains rps11, tscA, and most of chlN

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert:

Genes present:

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-71

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 19 (4.4 kb) in pUC8; contains psbH, psbN, and psbT

host strain: JM83
amp resistant

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 24 (3.3 kb) in pUC8, from CC-125 wild type; contains ycf12 and atpE

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 24 (3.3 kb)

Genes present: atpE

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab

Comment The 350 bp EcoRI-Bgl II subclone of this plasmid is a good probe for atpE.

P-73

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 26 (3.0 kb) in pUC8, from CC-125 wild type; internal to ORF2971

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 26 (3.0 kb)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-74

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 31 (1.4 kb) in pUC8, from CC-125 wild type; internal to rpoC2

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 31 (1.4 kb)

Genes present: rpoC2 (part)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-75

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 32 (1.3 kb) in pUC8, from CC-125 wild type; contains 3′ half of tufA

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 32 (1.3 kb)

Genes present: tufA (3′ end)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1983

P-76

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA PstI 4 (15.1 kb) in pBR322, from CC-125 wild type; contains rps2, rps18, ycf3, ycf4, rps9, psbE, rpoB and psbF

host strain: SK2267
tetracycline resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA PstI 4 (15.1 kb)

Genes present: rps2 (probably), atpF, psbE, rpoB, psbF

Vector: pBR322

Bacterial host strain: SK2267

Selectable marker: tet-r, amp-s

Origin: David Lamb in Boynton/Gillham lab, 1983

P-78a

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Orientation of P-78

P-78b

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA PstI 14 (3.8 kb) in pBR322, from CC-125 wild type; contains psbT and psbB

host strain: SK2267
tetracycline resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA PstI 14 (3.8 kb)

Genes present: psbB

Vector: pBR322

Bacterial host strain: SK2267

Selectable marker: tet-r, amp-s

Origin: David Lamb in Boynton/Gillham lab, 1983

P-82

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA PstI 19 (2.9 kb) in pBR322, from CC-125 wild type; contains rpoC1b

host strain: SK2267
tetracycline resistant

Boynton-Gillham laboratory, Duke University, 1983

Mitochondrial Bam-Sal fragment (5.6 kb) in pBR322, from CC-125 wild type; contains cox1, nd2, nd6, W, L6, L7, Q, and S1

host strain: SK2267
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: mitochondrial DNA BamHI-SalI (5.6 kb)

Genes present: cox1, nd2, nd6, W, L6, L5, L7, Q, S1

Vector: pBR322

Bacterial host strain: SK2267

Selectable marker: amp-r

Origin: Boynton/Gillham lab, 1983

P-85

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA EcoRI 22 (3.5 kb) in pUC8, from CC-125 wild type; contains nearly all of atpA, psbI, and most of cemA

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA EcoRI 22 (3.5 kb)

Genes present: atpA (most of gene; Eco site is near the 5′ end); psbI

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton-Gillham lab, 1983

Comment: This is a good probe for atpA.

P-86

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Chloroplast DNA BamHI 7 (11.6 kb) in pUC8, from CC-125 wild type; contains part of the Wendy transposon, petA, petD, and chlB

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ 137c

Insert: chloroplast DNA BamHI 7 (11.6 kb)

Genes present: petA, petD, chlB

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r

Origin: Boynton/Gillham lab, 1985

P-87

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1983

Nuclear rDNA unit, 3.6 kb BamH1 fragment in pUC8, from CC-125 wild type; contains part of 25S, 5.8S, and most of 18S rDNA

For map, see Marco and Rochaix (1980) Mol. Gen. Genet. 177, 715-723

host strain: JM83
amp resistant


Marco Y, Rochaix JD (1980) Organization of the nuclear ribosomal DNA of Chlamydomonas reinhardii. Mol Gen Genet 177:715-23

From Jean-David Rochaix to Boynton-Gillham laboratory, 1985

Chloroplast DNA EcoRI 27 (2.7 kb) from Rochaix’s cw15 strain in pCR1; contains most of psbD and the 5′ end of ORF2971

host strain: JM83
kanamycin resistant

 

More information

Strain: Rochaix cw15

Insert: chloroplast DNA EcoRI 27 (2.7 kb)

Genes present: psbD

Vector: pCR1 (11.4 kb)

Bacterial host strain: Uncertain

Selectable marker: kan-r

Origin: Jean-David Rochaix, 1985, as R3

P-99

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

Boynton-Gillham laboratory, Duke University, 1986

Chloroplast DNA EcoRI 36/37 (0.9 kb) in pUC8, from CC-125 wild type; contains wild-type 16S rRNA gene (3′ end);

host strain: JM83
amp resistant

 

More information

Strain: CC-125

Insert: EcoRI 36/37 (0.9 kb)

Genes present: 16S rRNA (3′ end)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1986

P-102


Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292