Search Results for pry127d

From Rosanna Young, Saul Purton lab, University College London, UK, October 2015

CrCD is a conditional negative selectable marker for use in the C. reinhardtii chloroplast that confers sensitivity to 5-fluorocytosine by converting it to toxic 5-fluorouracil (Young and Purton 2014). Plasmid pRY127d contains the crCD gene, with a C-terminal HA tag, inserted into the chloroplast expression vector pSRSapI. CrCD is a modified and codon-optimised version of the E. coli cytosine deaminase (codA) gene. The crCD gene in pRY127d uses the C. reinhardtii psaA exon 1 promoter and 5′ UTR and the C. reinhardtii rbcL 3′ UTR; the flanking regions in the construct target the crCD cassette downstream of psbH by homologous recombination.

This plasmid is called pCD in Young and Purton 2015.

Sequence (.docx file)