2 results found matching s: pwuca

From Rosanna Young, Saul Purton lab, University College London, UK, March 2017

pWUCA2 can be used to introduce the trnWUCA gene downstream of psbH in the C. reinhardtii chloroplast genome. trnWUCA is a synthetic tRNA that allows the readthrough of internal UGA codons. This plasmid also contains an empty expression cassette, where a gene of interest can be inserted (e.g. one with internal TGA codons).

100 μg/ml ampicillin in E. coli. Minimal medium for selection of restoration of psbH gene in C. reinhardtii (a psbH mutant recipient line must be used, e.g. TN72)

Sequence


Young RE & Purton S (2016) Codon reassignment to facilitate genetic engineering and biocontainment in the chloroplast of Chlamydomonas reinhardtii. Plant Biotechnol J. 14:251-60

From Rosanna Young, Saul Purton lab, University College London, UK, March 2017

pWUCA1 can be used to introduce the trnWUCA gene downstream of psaA exon 3 in the C. reinhardtii chloroplast genome using spectinomycin selection. trnWUCA is a synthetic tRNA that allows the readthrough of internal UGA codons.

100 μg/ml ampicillin in E. coli. 100 μg/ml spectinomycin for selection of aadA cassette in C. reinhardtii.

Sequence


Young RE & Purton S (2016) Codon reassignment to facilitate genetic engineering and biocontainment in the chloroplast of Chlamydomonas reinhardtii. Plant Biotechnol J. 14:251-60