Sueoka's high salt medium

    from Sueoka, N. (1960) Proc. Natl. Acad. Sci. USA 46, 83-91.

    Also known as HS or HSM, this is somewhat more economical than TAP to prepare.

     

    Make the following stock solutions:

      1. salts solution (also known as Beijerinck's solution)
       
      NH4Cl 100.0 g
      MgSO4 . 7H2O 4.0 g
      CaCl2 . 2H2O 2.0 g
      water to 1 liter 

      2. phosphate solution
       
      K2HPO4 288.0 g
      KH2PO4 144.0 g
      water to 1 liter 

      3. Hutner's trace elements (follow this link)

     

    To make the final medium, mix the following:

      5 ml solution #1 (salts)
      5 solution #2 (phosphate)
      1.0 ml solution #3 (trace elements)
      water to 1 liter

      Sodium acetate can be added at 1.2 g/liter (anhydrous) or 2.0 g/liter (hydrate).
      For solid medium, add 15 g agar per liter.
      Yeast extract 4.0 g/liter can also be added to solid medium.

      All these ingredients can be added before autoclaving.

      We don't recommend adding yeast extract to liquid media, since it encourages overgrowth of contaminating bacteria.

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