LOCUS       pRS426-ble-aphAV       10424 bp    DNA     circular     14-MAR-2012
DEFINITION  Yeast episomal vector pRS426 with URA3 marker, ATCC  77107.
KEYWORDS    ATCC.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|620050472|
COMMENT     VNTDBDATE|620585168|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRS426-ble-aphAVIII-gfp|
COMMENT     VNTAUTHORNAME|Sam Noor-Mohammadi|
COMMENT     VNTOAUTHORNAME|NCBI Entrez|
COMMENT     VNTOAUTHORTEL|(301)496-2475|
COMMENT     VNTOAUTHORFAX|(301)480-9241|
COMMENT     VNTOAUTHOREML|info@ncbi.nlm.nih.gov|
COMMENT     VNTOAUTHORAD1|National Center for Biotechnology Information|
COMMENT     VNTOAUTHORAD2|National Library of Medicine|
COMMENT     VNTOAUTHORAD3|Building 38A, Room 8N805|
COMMENT     VNTOAUTHORAD4|Bethesda, MD 20894, U.S.A.|
COMMENT     VNTREPLTYPE|Phagemid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
FEATURES             Location/Qualifiers
     CDS             8405..9208
                     /vntifkey="4"
                     /label=ura3
                     /note="orotidine-5'-phosphate"
     rep_origin      4645..5585
                     /vntifkey="33"
                     /label=pMB1
                     /note="origin of replication"
     CDS             complement(5588..6445)
                     /vntifkey="4"
                     /label=amp
                     /note="bla beta-lactamase, ampR ampicillin resistance"
     rep_origin      6578..7923
                     /vntifkey="33"
                     /label=2_MICRON
     CDS             10272..10279
                     /vntifkey="4"
                     /label=ble
     CDS             1..169
                     /vntifkey="4"
                     /label=ble
     intron          10280..10424
                     /vntifkey="15"
                     /label=Intron
     intron          170..314
                     /vntifkey="15"
                     /label=Intron
     CDS             315..527
                     /vntifkey="4"
                     /label=ble
     5'UTR           10064..10271
                     /vntifkey="52"
                     /label=RBCS2
     3'UTR           561..787
                     /vntifkey="50"
                     /label=RBCS2
     primer          9737..9753
                     /vntifkey="27"
                     /label=P249
     primer          10039..10088
                     /vntifkey="27"
                     /label=P250
     primer          complement(10039..10088)
                     /vntifkey="27"
                     /label=P251
     primer          complement(4658..4674)
                     /vntifkey="27"
                     /label=P254
     misc_feature    1169..1972
                     /vntifkey="21"
                     /label=AphAVIII
     misc_feature    2041..2202
                     /vntifkey="21"
                     /label=COP3'
     misc_feature    838..1168
                     /vntifkey="21"
                     /label=Beta2
     CDS             3026..3742
                     /vntifkey="4"
                     /label=gfp
     primer          3150..3173
                     /vntifkey="27"
                     /label=RT1
     primer          complement(3324..3347)
                     /vntifkey="27"
                     /label=RT2
     misc_feature    3743..4134
                     /vntifkey="21"
                     /label=3'
     misc_feature    2203..3025
                     /vntifkey="21"
                     /label=promoter/5'
BASE COUNT     2418 a      2581 c      2905 g      2520 t 
ORIGIN
        1 gatggccaag ctgaccagcg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt 
       61 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg 
      121 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tgagtcgacg 
      181 agcaagcccg gcggatcagg cagcgtgctt gcagatttga cttgcaacgc ccgcattgtg 
      241 tcgacgaagg cttttggctc ctctgtcgct gtctcaagca gcatctaacc ctgcgtcgcc 
      301 gtttccattt gcaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg 
      361 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct 
      421 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg 
      481 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactaaccg acgtcgaccc 
      541 actctagagg atcgatcccc gctccgtgta aatggaggcg ctcgttgatc tgagccttgc 
      601 cccctgacga acggcggtgg atggaagata ctgctctcaa gtgctgaagc ggtagcttag 
      661 ctccccgttt cgtgctgatc agtctttttc aacacgtaaa aagcggagga gttttgcaat 
      721 tttgttggtt gtaacgatcc tccgttgatt ttggcctctt tctccatggg cgggctgggc 
      781 gtatttgctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgtct 
      841 ttcttgcgct atgacacttc cagcaaaagg tagggcgggc tgcgagacgg cttcccggcg 
      901 ctgcatgcaa caccgatgat gcttcgaccc cccgaagctc cttcggggct gcatgggcgc 
      961 tccgatgccg ctccagggcg agcgctgttt aaatagccag gcccccgatt gcaaagacat 
     1021 tatagcgagc taccaaagcc atattcaaac acctagatca ctaccacttc tacacaggcc 
     1081 actcgagctt gtgatcgcac tccgctaagg gggcgcctct tcctcttcgt ttcagtcaca 
     1141 acccgcaaac atggtcgaga ttcgaagcat ggacgatgcg ttgcgtgcac tgcggggtcg 
     1201 gtatcccggt tgtgagtggg ttgttgtgga ggatggggcc tcgggggctg gtgtttatcg 
     1261 gcttcggggt ggtgggcggg agttgtttgt caaggtggca gctctggggg ccggggtggg 
     1321 cttgttgggt gaggctgaac ggctggtgtg gttggcggag gtggggattc ccgtacctcg 
     1381 tgttgtggag ggtggtgggg acgagagggt cgcctggttg gtcaccgaag cggttccggg 
     1441 gcgtccggcc agtgcgcggt ggccgcggga gcagcggctg gacgtggcgg tggcgctcgc 
     1501 ggggctcgct cgttcgctgc acgcgctgga ctgggagcgg tgtccgttcg atcgcagtct 
     1561 cgcggtgacg gtgccgcagg cggcccgtgc tgtcgctgaa gggagcgtcg acttggagga 
     1621 tctggacgag gagcggaagg ggtggtcggg ggagcggctt ctcgccgagc tggagcggac 
     1681 tcggcctgcg gacgaggatc tggcggtttg ccacggtgac ctgtgcccgg acaacgtgct 
     1741 gctcgaccct cgtacctgcg aggtgaccgg gctgatcgac gtggggcggg tcggccgtgc 
     1801 ggaccggcac tccgatctcg cgctggtgct gcgcgagctg gcccacgagg aggacccgtg 
     1861 gttcgggccg gagtgttccg cggcgttcct gcgggagtac gggcgcgggt gggatggggc 
     1921 ggtatcggag gaaaagctgg cgttttaccg gctgttggac gagttcttct gagggacctg 
     1981 atggtgttgg tggctgggta gggttgcgtc gcgtgggtga cagcacagtg tggacgttgg 
     2041 gatccggcaa gactggcccc gcttggcaac gcaacagtga gcccctccct agtgtgtttg 
     2101 gggatgtgac tatgtattcg tgtgttggcc aacgggtcaa cccgaacaga ttgatacccg 
     2161 ccttggcatt tcctgtcaga atgtaacgtc agttgatggt acggatccca cacacctgcc 
     2221 cgtctgcctg acaggaagtg aacgcatgtc gagggaggcc tcaccaatcg tcacacgagc 
     2281 cctcgtcaga aacacgtctc cgccacgctc tccctctcac ggccgacccc gcagcccttt 
     2341 tgccctttcc taggccaccg acaggaccca ggcgctctca gcatgcctca acaacccgta 
     2401 ctcgtgccag cggtgccctt gtgctggtga tcgcttggaa gcgcatgcga agacgaaggg 
     2461 gcggagcagg cggcctggct gttcgaaggg ctcgccgcca gttcgggtgc ctttctccac 
     2521 gcgcgcctcc acacctaccg atgcgtgaag gcaggcaaat gctcatgttt gcccgaactc 
     2581 ggagtcctta aaaagccgct tcttgtcgtc gttccgagac atgttagcag atcgcagtgc 
     2641 cacctttcct gacgcgctcg gccccatatt cggacgcaat tgtcatttgt agcacaattg 
     2701 gagcaaatct ggcgaggcag taggctttta agttgcaagg cgagagagca aagtgggacg 
     2761 cggcgtgatt attggtattt acgcgacggc ccggcgcgtt agcggccctt cccccaggcc 
     2821 agggacgatt atgtatcaat attgttgcgt tcgggcactc gtgcgagggc tcctgcgggc 
     2881 tggggagggg gatctgggaa ttggaggtac gaccgagatg gcttgctcgg ggggaggttt 
     2941 cctcgccgag caagccaggg ttaggtgttg cgctcttgac tcgttgtgca ttctaggacc 
     3001 ccactgctac tcacaacaag ccaaaatggc caagggcgag gagctgttca ccggtgtggt 
     3061 ccccatcctg gtggagctgg acggcgacgt gaacggccac aagttctccg tctccggcga 
     3121 gggtgagggt gacgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 
     3181 gctgcccgtg ccctggccca ccctggtcac caccctgacc tacggtgtgc agtgcttctc 
     3241 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgagggcta 
     3301 cgtgcaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 
     3361 caagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 
     3421 ggacggcaac atcctgggcc acaagctgga gtacaactac aactcccaca acgtgtacat 
     3481 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 
     3541 ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gcgatggccc 
     3601 cgtgctgctg cccgacaacc actacctgtc catccagtcc gccctgtcca aggaccccaa 
     3661 cgagaagcgc gaccacatgg tcctgctgga gttcgtcacc gctgccggca tcacccacgg 
     3721 catggacgag ctgtacaagt aagcgttctg gcagcagctg gaccgcctgt accatggaga 
     3781 agagctttac ttgccgggat ggccgatttc gctgattgat acgggatcgg agctcggagg 
     3841 ctttcgcgct aggggctagg cgaagggcag tggtgaccag ggtcggtgtg gggtcggccc 
     3901 acggtcaatt agccacagga ggatcagggg gaggtaggca cgtcgacttg gtttgcgacc 
     3961 ccgcagtttt ggcggacgtg ctgttgtaga tgttagcgtg tgcgtgagcc agtggccaac 
     4021 gtgccacacc cattgagaag accaaccaac ttactggcaa tatctgccaa tgccatactg 
     4081 catgtaatgg ccaggccatg tgagagtttg ccgtgcctgc gcgcgccccg gggggctccg 
     4141 tgtaaatgga ggcgctcgtt gatctgagcc ttgccccctg acgaacggcg gtggatggaa 
     4201 gatactgctc tcaagtgctg aagcggtagc ttagctcccc gtttcgtgct gatcagtctt 
     4261 tttcaacacg taaaaagcgg aggagttttg caattttgtt ggttgtaacg atcctccgtt 
     4321 gattttggcc tctttctcca tgggcgggct gggcgtattt ggcggtggag ctccagcttt 
     4381 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct 
     4441 gtgtgaaatt gttatccgct cacaattcca cacaacatag gagccggaag cataaagtgt 
     4501 aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc 
     4561 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 
     4621 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
     4681 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
     4741 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
     4801 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
     4861 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
     4921 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
     4981 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 
     5041 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
     5101 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
     5161 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
     5221 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 
     5281 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
     5341 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
     5401 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
     5461 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 
     5521 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
     5581 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 
     5641 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 
     5701 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 
     5761 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 
     5821 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
     5881 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 
     5941 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 
     6001 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 
     6061 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 
     6121 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
     6181 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 
     6241 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 
     6301 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 
     6361 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 
     6421 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
     6481 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
     6541 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt 
     6601 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat 
     6661 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc 
     6721 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc 
     6781 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat 
     6841 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct 
     6901 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca 
     6961 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa 
     7021 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt 
     7081 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa 
     7141 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta 
     7201 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac 
     7261 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata 
     7321 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt 
     7381 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga 
     7441 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa 
     7501 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag 
     7561 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa 
     7621 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg 
     7681 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac 
     7741 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc 
     7801 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg 
     7861 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc 
     7921 atactaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 
     7981 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 
     8041 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 
     8101 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 
     8161 gagagtgcac cataccacag cttttcaatt caattcatca tttttttttt attctttttt 
     8221 ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg 
     8281 aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg 
     8341 aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata 
     8401 aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc 
     8461 caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg 
     8521 taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa 
     8581 aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc 
     8641 attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa 
     8701 tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac 
     8761 gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga 
     8821 agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct 
     8881 atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt 
     8941 tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat 
     9001 tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac 
     9061 cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag gactatttgc 
     9121 aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata 
     9181 tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact 
     9241 aaactcacaa attagagctt caatttaatt atatcagtta ttaccctatg cggtgtgaaa 
     9301 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt 
     9361 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 
     9421 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 
     9481 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 
     9541 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 
     9601 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 
     9661 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 
     9721 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 
     9781 gctacagggc gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 
     9841 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 
     9901 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg 
     9961 cgcgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg 
    10021 tatcgataag cttgatatcg aattcctgca gcccggggga tccgccagaa ggagcgcagc 
    10081 caaaccagga tgatgtttga tggggtattt gagcacttgc aacccttatc cggaagcccc 
    10141 ctggcccaca aaggctaggc gccaatgcaa gcagttcgca tgcagcccct ggagcggtgc 
    10201 cctcctgata aaccggccag ggggcctatg ttctttactt ttttacaaga gaagtcactc 
    10261 aacatcttaa aatggccagg tgagtcgacg agcaagcccg gcggatcagg cagcgtgctt 
    10321 gcagatttga cttgcaacgc ccgcattgtg tcgacgaagg cttttggctc ctctgtcgct 
    10381 gtctcaagca gcatctaacc ctgcgtcgcc gtttccattt gcag 
//
