LOCUS       pTJ322                 11208 bp    DNA     circular     10-OCT-2011
DEFINITION  Yeast episomal vector pRS426 with URA3 marker, ATCC  77107.
KEYWORDS    ATCC.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|38836800|
COMMENT     VNTDBDATE|603647035|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTJ322|
COMMENT     VNTAUTHORNAME|Tyler Johannes|
COMMENT     VNTAUTHOREML|johannes@utulsa.edu|
COMMENT     VNTOAUTHORNAME|NCBI Entrez|
COMMENT     VNTOAUTHORTEL|(301)496-2475|
COMMENT     VNTOAUTHORFAX|(301)480-9241|
COMMENT     VNTOAUTHOREML|info@ncbi.nlm.nih.gov|
COMMENT     VNTOAUTHORAD1|National Center for Biotechnology Information|
COMMENT     VNTOAUTHORAD2|National Library of Medicine|
COMMENT     VNTOAUTHORAD3|Building 38A, Room 8N805|
COMMENT     VNTOAUTHORAD4|Bethesda, MD 20894, U.S.A.|
COMMENT     VNTREPLTYPE|Phagemid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
FEATURES             Location/Qualifiers
     CDS             1267..2070
                     /vntifkey="4"
                     /label=ura3
                     /note="orotidine-5'-phosphate"
     rep_origin      complement(2201..2657)
                     /vntifkey="33"
                     /label=f1\origin
     rep_origin      8715..9655
                     /vntifkey="33"
                     /label=pMB1\origin
                     /note="origin of replication"
     CDS             10648..785
                     /vntifkey="4"
                     /label=2\micron
     misc_signal     2875..8376
                     /vntifkey="87"
                     /label=cpDNA
     CDS             9656..10516
                     /vntifkey="4"
                     /label=amp
BASE COUNT     3228 a      2277 c      2471 g      3232 t 
ORIGIN
        1 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc 
       61 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc 
      121 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca 
      181 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg 
      241 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg 
      301 gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg 
      361 aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct 
      421 agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa 
      481 aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt 
      541 tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt 
      601 acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc 
      661 ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc 
      721 tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga 
      781 tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 
      841 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 
      901 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 
      961 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 
     1021 ctgagagtgc accataccac agcttttcaa ttcaattcat catttttttt ttattctttt 
     1081 ttttgatttc ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa 
     1141 cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca 
     1201 tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga 
     1261 taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct 
     1321 gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt 
     1381 cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta 
     1441 aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag 
     1501 gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt 
     1561 aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt 
     1621 acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa 
     1681 gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc 
     1741 ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt 
     1801 gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg 
     1861 attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga 
     1921 accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt 
     1981 gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca 
     2041 tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata 
     2101 ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga 
     2161 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 
     2221 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     2281 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     2341 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     2401 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     2461 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     2521 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     2581 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     2641 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 
     2701 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 
     2761 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 
     2821 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgagagtggg 
     2881 aaagtataat acgccgactc ctgccattaa cagtagcagg atgaagacat agtccatgcc 
     2941 tttacgaaag taaaggggtt agttttaaag accgcaagtt ttattcggct ttaaaatttc 
     3001 atgcgtgaga cagtttggtc catatccggt gtaggcgtta gagcattgag agtagccttt 
     3061 catagtacga gaggacctga aaggacatgc caattgtgta ccagttctca ttccaatggg 
     3121 aaacgctggg tagctacgca tggatagata actgctgaaa gcatctaagt aggaagctaa 
     3181 actcaagatg agtgctctct aaggccgcgg ctagacaagc cgttatatag gtatcaggtg 
     3241 tacagtcagc aatggcttta gccgagatat actaaaggcc gtttgatttt gaccttataa 
     3301 tataattaca taaccccttg cgggtaacta tcgtttatga gctaagctaa gcttagttta 
     3361 actagcatct cacagagatg cagttaacct tggtgctctt tgctcagttg gacccacacc 
     3421 aatccatccc gaacttggtt gtgaaaaagc tgaggggact gaagaacttt acgggtcgcc 
     3481 gtctggaatc tcagttctag tgctagggtt aaaaaatatt taaataatgc ctaggaaacc 
     3541 aaagaagctc caacctccgt tggagcctcc actcccctcc ccctacggga catccccctc 
     3601 cccctacggg attatttata taaccaccga aggacgtccc cttacgggca aataaatttt 
     3661 agtgtgccag tagcaaaagt attaatatcg tatataaata tcctgccaac tgcctaggca 
     3721 agtaaactta gggattttaa tgctccgttt aggacgtccc cttccccttc gggacgtccc 
     3781 cttacgggaa tataaatatt agtggacgtc cccttcgggc aaatgaattt tagtggcagt 
     3841 tgcctgccaa ctgccgatat ttatatactg cgataaactt tagttgcccg aaggggttta 
     3901 catacaattt atttattgta ccactgccac taatatttat attcccgtaa ggggacgtcc 
     3961 tccttcccct tcgggcaagt aaacttagga gtatgtaaac ctgctagcgc agcaaataaa 
     4021 ttttattcta agtttacttg cccgaagggg aaggaggaag caggcagttg cctccttccc 
     4081 cttccccttc gggacgtccc cttcgggatt ttaatgctcc gttaggaggc aaataaattt 
     4141 tagtggcagt tgcctgccaa ctgcctcctt cggagtatta aattaggacg tccccttacg 
     4201 ggaatataaa tattagtggc agttgcctgc caactgcctc cttcggagta ttaaaatagg 
     4261 atgttaatac tgcggacagg cagtggcggt accactgcca ctggcgtcct ccttcggagt 
     4321 atgtaaacat tctatattta tatactgcga taaactttag ttgcccgaag gggtttacat 
     4381 acaatttatt tattgtacca ctgccactaa tatttatatt cccgtaaggg gacgtcctcc 
     4441 ttccccttcg ggcaagtaaa cttaggagta tgtaaacctg ctagcgcagc aaataaattt 
     4501 tattctaagt ttacttgccc gaaggggaag gaggaagcag gcagtggtgg taccactgcc 
     4561 actaaaattt atttacccga aggggacgtc cttccccttc cccttcggga cgtcagtggc 
     4621 agttgcctgc caactgccta atataaatat tgggcaagta aacttaggag tatataaata 
     4681 taggatttta atactccgaa ggaggcagtt ggcaggcaac tgccactgac gtcacgtaag 
     4741 gggaagggga caaatttatt tattgtcccg taagggaaag tcgtggagta tttaatacag 
     4801 ctaaaagact taaagaaact ttagatttag ctataaaact taaagcgaca ggtacttccg 
     4861 aaacggtggt tattccaggc caaacttatg aaatgcaagt accatcagat attgctagca 
     4921 tggatacgga tccagcgata ttaaagttat tagaagaaaa taaaactagt ccatatccaa 
     4981 ttccaattat tccaattgaa actatttatg ggttatttac taatatagct gaagattaat 
     5041 tttttttaaa aaaatattta atgcaattgc aataataata aatagcatta aatatgtaaa 
     5101 ttttaattaa ctaaaagacc taatccagcc ataatataaa cgaaagtgaa taaagtgata 
     5161 tatatctgaa ccgatttgat agcgacgaaa ggctaataac gttgtagcaa ttaaacgtct 
     5221 acatatggcg aaaaaaaaat tggattaacc ttcggtaagt aaagcgatta accttcagta 
     5281 agttaagcaa ttaaccttcg gtaagtaaag cgattaacct tcagtaagtt aagcaattaa 
     5341 ccttcggtaa gtaaagcgat taaccttcgg taagtaaagc gattaacctt cggtaagtta 
     5401 agcaattaac ggtcgataga ctaataaaaa tttatgtatt taaaccttgt ctaacagcgt 
     5461 ataaatataa cagcgcttat ataaatgtaa gcccaggctg tcacgaagtg attattgacc 
     5521 actaggaagg acacaggacg tcccataaga tccacgaagg catttacctt cggtaagata 
     5581 ataaacctcg atgtagaggc cataagagct caaatttaca tatcgtatac gacagattta 
     5641 catatcgtag acgacaaata cttttttatt tagtcagtca cagaacctac caagacgcca 
     5701 gtgaacgtca cacagccact aaaatttatt taccgaaggg gacgtcctaa tataaatatt 
     5761 gggcaagtaa acttagctat ttttatagga cgaaacgtgt ggttctcacc atctgtgtga 
     5821 aacgaagccg atggtttttt gtggcagttg ccatcggcta agaagttctt cggagtatat 
     5881 aaatatagga tgttaatact gcggagccag tggtggtacc actgcctcct tccccttctt 
     5941 cgggacgtct tcgggtaaat aaattttagt cgagttgcct gccaactgcc tttcgagtat 
     6001 aaaatacgag tggtggtcca ctgccactga cgtcctaata taaatattgg gcaagtaaac 
     6061 ttagcatgtt tacatactcc gaaggaggac gtcttacgga atataaatat tagtcgagtg 
     6121 gtaccgccac tgcctagtat ataaatatcg gcagttggca ggatatttat atactccgaa 
     6181 ggaacttgtt agccgatagg cgaggcaact gccactaaaa tttatttgcc tcctaacgga 
     6241 gcattaaaat ccctaagttt acttgcccgt aaggggaagg ggacgtccac taatatttat 
     6301 attaggcagt tggcaggcaa caataaatac atttgtcccg taaggggacg tcctgccaac 
     6361 tgcctatggt agctattaag tatatatata tgaaaagtgt gtataaacta aaataaacca 
     6421 ggtatggtta accagattta ttttagttta aaaaaaaatt agttgtttga gctagagtta 
     6481 gttgaagcta agtctagagg gaagttgtga gcgttacgct cgtgcattac ttccatacct 
     6541 aagttagcac ggttgatgat gtctgcccaa gtgtttagta cacgaccttg tgagtctact 
     6601 actgattggt tgaagttgaa accgtttaag ttgaatgcca tagttgataa acctaaagca 
     6661 gtgaaccaaa taccgattac cggccaagca gctaagaaga agtgtaatga acgagagttg 
     6721 ttgaaagaag cgtattggaa gattagacga ccaaagtaac catttaatat agactaaaaa 
     6781 cctttttaat ctactggtcg tcgtccacct ttttcaaggt gggttggact atatcaccat 
     6841 ctaaaattta aaccatttta gatgccgggc gctagtgagc tattattgtt gggactcaag 
     6901 ctctagtctc tgaacgttct agggaagcaa tgcccatccc tagcttcgct gcaggttgcc 
     6961 atattattac aaaaaacgta aaaaggttta aaacgttatc cagccatatg cagaaggtcg 
     7021 ttgtttttta attacacgag ccagatttga agtaactgta cttgtttttg ctctttctaa 
     7081 aacttcttta tctttacatg gtggtaaagc ttctaaaagt ttttgaacta gctgcggaag 
     7141 cgtaaacaat ctgtttggtt gaaggtttag ttcagttccg gattgtaaat gtttccaacg 
     7201 tgaaccttcg taaagagctt ttaatacggg ttggctcatt tttgtacttt gttttaaatc 
     7261 tttttcgtga ctttttacta gaccaccaat acgtccacct ttagaagaag cgccttcggc 
     7321 atagatacca gtacgttctc ttaaacgcgt ttcgttggct atttggtttc ctcgaagacg 
     7381 tcgttcaact agctcagaag gattagttct gaaacgaatt gcttggtgat caccagcttc 
     7441 gttatatact aaagcacgta atcggtgagc ttccatatgg tcttctggtg ttaattttat 
     7501 taaattccag cgtttatgtg gaccaccggt gtgtttagga ataatatgat ggttttcagt 
     7561 atattttcct ataactggat ggtcaatccg ttggagtata aaattgttat aaacgtttgg 
     7621 actttctttc agaaaaattt ttaaatcttc tttattagaa atatgtgcat atgctaaacc 
     7681 tttttgtaag tgtttttgtt gtttatattc atttaaaacg gcctgtttag aggcctcttc 
     7741 aggagatttt gtacgaattt tacctttcca gttttcactt gttgtttttg ctttacattg 
     7801 aggacaacct gttttacgag cgttctgata agaacgtgct gttgttgtaa agctggtttt 
     7861 acaagtttta caaaaaattg taattgaacc ttgagaaggt gtttggatgt tactaagaga 
     7921 aattagttcg tgattacgaa cctgagctat attagcataa aattgtattg ttttttttgt 
     7981 agtcataaag ggatttaatg ttatttacac ccgtaagggg aaaaatttta tatttttatt 
     8041 aagcaaactt gattattcaa actttaaaat aatatttatt ttattataac aaagttgtaa 
     8101 aaaaaaaaca agtccccttt atttatagac cttaaaacac aacaatgttg ctattacctt 
     8161 cggtcatacc ttttggtcaa ccttcgtgac atattgtaaa gtgtcatcga acgtcatcga 
     8221 acgtcattga aactcatcaa atgtcgtccc cttcggggct gcaaagcagc aaaactcccc 
     8281 aaatgttcct gaagcttagt gaaggttagc tcgtataatt tcttaaaaac atatttaacg 
     8341 cagttttaac tgttaaaact tcgtttttct tcgtttgaat tctgaaatcc ttccctcgat 
     8401 cccgaggttg ttgttattgt tattgttgtt gttgttcgag ctccagcttt tgttcccttt 
     8461 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 
     8521 gttatccgct cacaattcca cacaacatag gagccggaag cataaagtgt aaagcctggg 
     8581 gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
     8641 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     8701 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     8761 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     8821 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     8881 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     8941 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     9001 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     9061 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     9121 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     9181 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     9241 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     9301 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     9361 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     9421 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     9481 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     9541 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     9601 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     9661 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     9721 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     9781 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     9841 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     9901 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     9961 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
    10021 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
    10081 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
    10141 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
    10201 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
    10261 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
    10321 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
    10381 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
    10441 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
    10501 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
    10561 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
    10621 gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa 
    10681 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa 
    10741 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa 
    10801 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta 
    10861 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt 
    10921 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt 
    10981 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc 
    11041 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac 
    11101 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag 
    11161 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtg 
//
