Chlamy Growth Media

Adapted from various in-lab sources

Stock Solutions


For 1L of media

TAP Media


Stock Solutions

Phosphate Buffer II
Component For 100 ml
K2HPO4 10.8 g
KH2PO4 5.6 g
Solution A
Component for 500 ml
NH4Cl 20 g
MgSO4·7H2O 5 g
CaCl2·2H2O 2.5 g

Hutner's Trace Metals

  1. Dissolve 50g of acid free EDTA in 250 ml of ddH2O. Heat to dissolve.
  2. Dissolve the following, one by one, in order, heating to approximately 100° in 550 ml ddH2O.
  3. Mix the two solutions together. The resulting solution should be blue-green.
  4. Heat to 100°. Cool slightly, but don't let the temperature drop below 80°-90°.
  5. Adjust pH to 6.5 to 6.8 with 20% KOH (approximately 83 ml). Don't let the temperature drop below 70 ° until after the pH is adjusted.
  6. Make up to 1L and let stand in a 2L Erlenmeyer flask, stoppered loosly. The colour should change from green to purple.
  7. Remove the rust-coloured precipitate by filtering, with suction, through 3 layers of Whatman #1 filter paper in a Buchner funnel. Repeat until no more precipitate is seen on the filter paper.
  8. Store in a brown bottle at 4°.

Gorman, D.S. and R.P. Levine (1965). Proc. Natl. Acad. Sci. USA 54:1665-1669.
Hutner, et al. (1950). Methods in Enzymology, 23:68.
Sager, R. and S. Granick (1953). Ann. N.Y. Acad. Sci. 56:831-838.
This page is, as always, Copyright (c) 1998 by Craig D. Amundsen. All rights reserved.