CC-5394 ∆MAT3-A5 mt+ [PH57]
$30.00
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University-Berlin, February 2018
This is a MAT3 disruption strain, generated with CRISPR/Cas9. MAT3 shows strong homology to the animal retinoblastoma cancer gene.
Background strain CC-125
Nuclease (Sp)Cas9 as ribonucleoprotein (RNP)
Marker pAphVII (pPH360)
Target gene MAT3, Cre06.g255450
Target sequence GCTGAAGGAGAACTCGGAAACGG (Exon 3)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P (2017) Targeting of photoreceptor genes via zinc-finger nucleases and CRISPR/Cas9 in Chlamydomonas reinhardtii. Plant Cell 29, Issue 10