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CC-5446 ∆Phot ∆UVR8-B3 mt+ [PH130]

Name: CC-5446 ∆Phot ∆UVR8-B3 mt+ [PH130]
SKU: CC-5446
Price: 30.00 USD
Availability: InStock

$30.00

Deposited by Simon Kelterborn, Peter Hegemann lab, Humboldt University-Berlin, December 2018

This is a PHOT/ UVR8 double disruption strain, generated with CRISPR/Cas9.

Background strain: CC-5392 ∆PHOT-B5 [PH15] (based on CC-125)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: UVR8, Cre05.g230600
Target sequence: CGGCGTCACAGTGACGAGTGTGG (Exon 6)

CC-5392 ∆PHOT-B5 [PH15] strain info:
Marker: pAphVII (pPH360)
Target gene: PHOT, Cre03.g199000
Target sequence: GCGCATCCTCAACTACACCAAGG (Exon 6)

Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de

This is an unpublished strain. Please contact ph@chlamy.de before using it.

CC-5446 ∆Phot ∆UVR8-B3 mt+ [PH130]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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