* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-6304 lf4; CDKL5-GFP mt+ [T3D2]

Name: CC-6304 lf4; CDKL5-GFP mt+ [T3D2]
SKU: CC-6304
Availability: InStock

$30.00

From George B. Witman, UMass Chan Medical School, Worcester, MA, September 2025

Background: CC-4768 (lf4-9 mt+)
Origin: Made by TIM-tagging LF5 with sfGFP tag right in front of the stop codon in CC-4768.
Culture maintenance: Can be maintained on Tris-acetate-phosphate (Gorman and Levine, 1965) agar plates or slants, or grown in liquid modified Medium I (Sager and Granick, 1953; Witman, 1986).
Comment: In this strain, the endogenous LF5 gene is tagged with sfGFP at the C-terminus right in front of the stop codon. Cells have a long-flagella phenotype due to the lf4-9 allele.

Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.

Chlamydomonas Resource Center

Chlamydomonas Resource Center

CC-6304 lf4; CDKL5-GFP mt+ [T3D2]

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