* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-6305 lf5; CDKL5-GFP-TG mt- [C12]

Name: CC-6305 lf5; CDKL5-GFP-TG mt- [C12]
SKU: CC-6305
Availability: InStock

$30.00

From George B. Witman, UMass Chan Medical School, Worcester, MA, September 2025

Background: CC-4560 (lf5-2 mt+)
Origin: Made by transforming CC-4560 cells with exogenous wild-type LF5 gene tagged with sfGFP right in front of the stop codon.
Culture maintenance: Can be maintained on Tris-acetate-phosphate (Gorman and Levine, 1965) agar plates or slants, or grown in liquid modified Medium I (Sager and Granick, 1953; Witman, 1986).
Comment: An exogenous copy of the wild-type LF5 gene tagged with sfGFP at the C-terminus right in front of the stop codon is incorporated into the genome of this strain. Thus, the long-flagella phenotype caused by the lf5-2 allele is rescued by the wild-type transgene. CC-4560 is reported to be mt+ but our analysis indicates that it is mt-.

Chlamydomonas Resource Center

Chlamydomonas Resource Center

CC-6305 lf5; CDKL5-GFP-TG mt- [C12]

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