* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-6307 lf5; CDKL5-Y166F-GFP-TG mt- [Y166F1]
$30.00
From George B. Witman, UMass Chan Medical School, Worcester, MA, September 2025
Origin: Made by transforming CC-4560 cells with exogenous mutant LF5Y166F gene tagged with sfGFP right in front of the stop codon.
Culture maintenance: Can be maintained on Tris-acetate-phosphate (Gorman and Levine, 1965) agar plates or slants, or grown in liquid modified Medium I (Sager and Granick, 1953; Witman, 1986).
Comment: An exogenous mutant LF5-Y166F gene tagged with sfGFP at the C-terminus right in front of the stop codon is incorporated into the genome of this strain. The mutant LF5-Y166F transgene is not able to rescue the long-flagella phenotype caused by the lf5-2 allele. CC4560 is reported to be mt+ but our analysis indicates that it is mt-.