* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-6308 lf5; CDKL5-S162A,T164A,Y166A-GFP-TG mt- [3AB5]

Name: CC-6308 lf5; CDKL5-S162A,T164A,Y166A-GFP-TG mt- [3AB5]
SKU: CC-6308
Availability: InStock

$30.00

From George B. Witman, UMass Chan Medical School, Worcester, MA, September 2025

Background: CC-4560 (lf5-2 mt+)
Origin: Made by transforming CC-4560 cells with exogenous mutant LF53A gene (S162, T164, and Y166 at the activation loop all mutated to A) tagged with sfGFP right in front of the stop codon.
Culture maintenance: Can be maintained on Tris-acetate-phosphate (Gorman and Levine, 1965) agar plates or slants, or grown in liquid modified Medium I (Sager and Granick, 1953; Witman, 1986).
Comment: An exogenous mutant LF53A gene tagged with sfGFP at the C-terminus right in front of the stop codon is incorporated into the genome of this strain. The mutant LF53A transgene is not able to rescue the long-flagella phenotype caused by the lf5-2 allele. CC4560 is reported to be mt+ but our analysis indicates that it is mt-.

Chlamydomonas Resource Center

Chlamydomonas Resource Center

CC-6308 lf5; CDKL5-S162A,T164A,Y166A-GFP-TG mt- [3AB5]

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