CC-6309 g1; CDKL5-GFP-TG mt+ [g1CDKL5F1]

$30.00

From George B. Witman, UMass Chan Medical School, Worcester, MA, September 2025

Background: CC-5415 (Witman g1)
Origin: Made by transforming wild-type CC-5415 (Witman g1) cells with exogenous wild-type LF5 gene tagged with sfGFP right in front of the stop codon.
Culture maintenance: Can be maintained on Tris-acetate-phosphate (Gorman and Levine, 1965) agar plates or slants, or grown in liquid modified Medium I (Sager and Granick, 1953; Witman, 1986).
Comment:  An exogenous wild-type LF5 gene tagged with sfGFP at the C-terminus right in front of the stop codon is incorporated into the genome of this strain. The cells express both GFP-tagged wild-type LF5 from the exogenous gene and untagged wild-type LF5 from the endogenous gene. Cells have a wild-type phenotype.