* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-6360 kea1-1 mt-

Name: CC-6360 kea1-1 mt-
SKU: CC-6360
Availability: InStock

$30.00

From Tobias Wunder, Hans-Henning Kunz lab, Ludwig Maximilian University-Munich, April 2026

This is a knock-out generated by CRISPR/Cas9 (following Greiner et al. 2017) targeting exon 19 of Cre04.g220200, inserting a stop-codon–containing sequenced. Co-transformation of pBC1 (pJR38, Neupert et al., 2009) conferring paromomycin resistance.

Knockout of CrKEA1 (Cre04.g220200) is a predicted chloroplast envelope K⁺/H⁺ antiporter and homolog of Arabidopsis AtKEA1/AtKEA2, verified by immunoblot. Simply called kea1 (and not kea1-1) in Wunder et al. 2026 (bioRxiv). The exact nature of the knock-out insertion and more described in Wunder et al. 2026.

https://www.biorxiv.org/content/10.64898/2025.12.03.692059v2

Chlamydomonas Resource Center

Chlamydomonas Resource Center

CC-6360 kea1-1 mt-

Products

Support

Cart