pCC1-23
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
TU short name: PatpA·aadA·TrbcL
TU full name: pL1-1F-atpApro::aadA::TrbcL
Host strain: DH5α
Selection in E. coli: Ampicillin (100µg/ml)
Type: 1F
Original pl1: pICH47732
Function: Resistance in Chlamy chloroplast to Spectinomycin
Source of the module: Melero-Cobo et al., 2025
Designer/ Builder: Melero-Cobo & Gallemí
Fusion 5′ lvl2: TGCC
Fusion 3′ lvl2: GCAA
Comment: This module was not obtained by Golden Gate. Refer to Melero-Cobo et al. for further details.
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.