* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.

pCM0_TB129

Name: pCM0_TB129
SKU: M-132 (spec)
Price: 30.00 USD
Availability: InStock

$30.00

From Thomas Baier, Olaf Kruse lab, Bielefeld University, August 2021

Host strain: DH5α
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): A1_B2
Brick name: P_AßSAP(i)(Pro + 5’UTR)
Brick type: promoter + 5’UTR
Brick function: Synthetic HSP70A/ßtub promoter, which drives 4xstronger expression compared to pAR when transgene is expressed to the cytosol
Source of Brick: Einhaus et al. 2021
Acceptor plasmid: pICH41295
Designer / Builder: A. Einhaus and T. Baier

Sequence

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/

pCM0_TB129

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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