From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 10

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

Phenotype: bigger CVs

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 13

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 17

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

Phenotype: bigger CVs, longer CV cycle

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM su


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 5

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

Phenotype: bigger CVs

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 4

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 5

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 2

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 3

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 17

From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016

Phenotype: longer CV cycle, smaller CVs

This strain cannot survive under osmotic conditions below 140 mosM.

Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose


Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895

Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.


  • Locus:
  • Chromosome:
  • 16

From Lynne Quarmby, Simon Fraser University, March 2017

Phenotype: impaired flagellar autotomy


Hilton LK, Meili F, Buckoll PD, Rodriguez-Pike JC, Choutka CP, Kirschner JA, Warner F, Lethan M, Garces FA, Qi J, Quarmby LM (2016) A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel. G3 (Bethesda). 6:3409-3418


  • Locus:
  • ADF1
  • Chromosome:
  • 9

From Lynne Quarmby, Simon Fraser University, March 2017

Phenotype: impaired flagellar autotomy


Hilton LK, Meili F, Buckoll PD, Rodriguez-Pike JC, Choutka CP, Kirschner JA, Warner F, Lethan M, Garces FA, Qi J, Quarmby LM (2016) A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel. G3 (Bethesda). 6:3409-3418

From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017


Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63

From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017


Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63

From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017


Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63

From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017

This mutant should be maintained in low light (~50 uE or less) or TAP in the dark.


Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63

From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017


Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63

From Steven King, University of Connecticut Health Center, June 2017

This is a flagellated empty vector control strain used in analysis of amiRNA strains that reduce levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850).


Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

From Steven King, University of Connecticut Health Center, June 2017

This is a flagellated empty vector control strain used in analysis of amiRNA strains that reduce levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850).


Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

From Steven King, University of Connecticut Health Center, June 2017

This is a flagellated empty vector control strain used in analysis of amiRNA strains that reduce levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850).


Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

From Steven King, University of Connecticut Health Center, June 2017

This is an amiRNA strain with reduced levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). This strain exhibits a weak knockdown of PAM expression resulting in a mixture of flagellated and unflagellated cells. For a strong knockdown of PAM expression use PAM amiRNA2 #3 or #8


Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

From Steven King, University of Connecticut Health Center, June 2017

This is an amiRNA strain with reduced levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). This strain exhibits a weak knockdown of PAM expression resulting in a mixture of flagellated and unflagellated cells. For a strong knockdown of PAM expression use PAM amiRNA2 #3 or #8


Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

From Steven King, University of Connecticut Health Center, June 2017

This is an amiRNA strain with reduced levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). This strain exhibits a strong knockdown of PAM expression to ~30% of control levels resulting in aflagellate cells.


Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

From Steven King, University of Connecticut Health Center, June 2017

This is an amiRNA strain with reduced levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). This strain exhibits a strong knockdown of PAM expression to ~10% of control levels resulting in aflagellate cells.


Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

From Steven King, University of Connecticut Health Center, June 2017

This CLiP library strain contains an insertion in the gene for peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). The insertion occurs in the region encoding the cytosolic C-terminal domain, and results in deletion of the last 75 residues and incorporation of an 18-residue sequence derived from the insertion cassette. This strain exhibits enhanced PAM enzyme activity.


Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

From Martin Spalding, Iowa State University, June 2017

21gr (CC-1690) was initially crossed to CC-1691 to obtain a mt- strain, and then backcrossed to 21gr (CC-1690) fifteen times. This is an mt- strain from the final backcross.

From Martin Spalding, Iowa State University, June 2017

This starchless mutant arose from a TALEN targeted mutagenesis experiment using 21gr (CC-1690). The mutation in the STA6 gene is a single base deletion at the intron 3/exon 4 border yielding the following sequence: GATGCAGaCGGTGCT where the lowercase ‘a’ is the deleted base. To obtain a minus mating type, the initial mutant was crossed to CC-1691.  A starchless mt- progeny lacking the TALEN transgene was subsequently backcrossed to 21gr (CC-1690) thirteen times. This strain is a mt- progeny from the final cross.


  • Locus:
  • STA6 [AGP4]
  • Chromosome:
  • 3

From Martin Spalding, Iowa State University, June 2017

This starchless mutant arose from a TALEN targeted mutagenesis experiment using 21gr (CC-1690). The mutation in the STA6 gene is a single base deletion at the intron 3/exon 4 border yielding the following sequence: GATGCAGaCGGTGCT where the lowercase ‘a’ is the deleted base. To obtain a minus mating type, the initial mutant was crossed to CC-1691. A starchless mt- progeny lacking the TALEN transgene was subsequently backcrossed to 21gr (CC-1690) thirteen times. This strain is a mt+ progeny from the final cross.


  • Locus:
  • STA6 [AGP4]
  • Chromosome:
  • 3