From Hiroaki Ishikawa, Wallace Marshall Lab, UCSF, March 2018

This mutant was created by crossing pf18 (CC-1036) to fla3 KAP-GFP (CC-4296). This strain has the mutant pf18 and fla3 alleles.


Mueller J, Perrone CA, Bower R, Cole DG, Porter ME (2005) The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell 16:1341–1354

Engel BD, Lechtreck KF, Sakai T, Ikebe M, Witman GB, Marshall WF (2009) Total Internal Reflection Fluorescence (TIRF) Microscopy of Chlamydomonas Flagella. Methods Cell Biol 93:157-177

Engel BD, Ludington WB, Marshall WF (2009) Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model. J Cell Biol 187:81–89


  • Locus:
  • PF18, FLA3
  • Chromosome:
  • 02, 10

From Hiroaki Ishikawa, Wallace Marshall Lab, UCSF, March 2018

This mutant was created by crossing pf18 with IFT27-GFP. IFT27-GFP is weakly expressed.


Qin H, Diener DR, Geimer S, Cole DG, Rosenbaum JL (2004) Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body. J Cell Biol 164:255–266

Engel BD, Lechtreck KF, Sakai T, Ikebe M, Witman GB, Marshall WF (2009) Total Internal Reflection Fluorescence (TIRF) Microscopy of Chlamydomonas Flagella. Methods Cell Biol 93:157-177

Engel BD, Ludington WB, Marshall WF (2009) Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model. J Cell Biol 187:81–89


  • Locus:
  • PF18
  • Chromosome:
  • 02

From Emily Hunter, Sale laboratory, Emory University, March 2018

This strain is transformant # 13-13-12. This cell-type has an ida3+ background and has been transformed with an IDA3-HA vector. The HA-tag is embedded within the second exon of IDA3. This cell-type is Hygromycin resistant.


Hunter EL, Lechtreck K, Fu G, Hwang J, Lin H, Gokhale A, Alford LM, Lewis B, Yamamoto R, Kamiya R, Yang F, Nicastro D, Dutcher SK, Wirschell M, Sale WS (2018) The IDA3 adapter, required for IFT transport of I1 dynein, is regulated by ciliary length. Mol Biol Cell. Feb 21. [Epub ahead of print] PubMed PMID: 29467251


  • Locus:
  • IDA3
  • Chromosome:
  • 03

From Emily Hunter, Sale laboratory, Emory University, March 2018

This strain is transformant # 15-3-14. This cell-type has an ida3+ background and has been transformed with an IDA3-mNeonGreen vector. The NG-tag is embedded within the second exon of IDA3. This cell-type is hygromycin resistant.

Note: 15-3-14 was used for imaging in Hunter et al., 2018.


Hunter EL, Lechtreck K, Fu G, Hwang J, Lin H, Gokhale A, Alford LM, Lewis B, Yamamoto R, Kamiya R, Yang F, Nicastro D, Dutcher SK, Wirschell M, Sale WS (2018) The IDA3 adapter, required for IFT transport of I1 dynein, is regulated by ciliary length. Mol Biol Cell. Feb 21. [Epub ahead of print] PubMed PMID: 29467251


  • Locus:
  • IDA3
  • Chromosome:
  • 03

From Emily Hunter, Sale laboratory, Emory University, March 2018

This cell-type contains the ida7 mutation, the ida3 mutation, and a wild-type copy of IDA3-NG. This cell line is Hygromycin resistant. This cell-line was obtained by mating ida7 x ida3; IDA3-NG followed by tetrad analysis. Susan Dutcher assisted in the mating and isolation of this cell-type. This cell-type prefers L-media.


Hunter EL, Lechtreck K, Fu G, Hwang J, Lin H, Gokhale A, Alford LM, Lewis B, Yamamoto R, Kamiya R, Yang F, Nicastro D, Dutcher SK, Wirschell M, Sale WS (2018) The IDA3 adapter, required for IFT transport of I1 dynein, is regulated by ciliary length. Mol Biol Cell. Feb 21. [Epub ahead of print] PubMed PMID: 29467251


  • Locus:
  • IDA3, IDA7
  • Chromosome:
  • 03, 16

From Sunghoon Jang, Youngsook Lee Lab, POSTECH-South Korea, April 2018

This strain has a CC-503 cw92 mt+ background and has been transformed with an AphVII cassette. The cassette is embedded within the fourth exon of MEX1. This strain is Hygromycin resistant.


Jang, Sunghoon, Yasuyo Yamaoka, Dong-hwi Ko, Tomokazu Kurita, Kyungyoon Kim, Won-Yong Song, Jae-Ung Hwang, Byung-Ho Kang, Ikuo Nishida, and Youngsook Lee. "Characterization of a Chlamydomonas reinhardtii mutant defective in a maltose transporter." Journal of plant biology 58, no. 5 (2015): 344-351


  • Locus:
  • MEX1
  • Chromosome:
  • 12

From George Witman, UMASS Medical School, August 2018

This strain, which shows strong negative phototaxis and whose nucleus is easily transformed, is the parent strain for many of the insertional mutants generated in the Witman lab. It is a cross of nit1-305 (from J. Rosenbaum) to CC-124.


Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol. 131:427-40


  • Locus:
  • NIT1, AGG1
  • Chromosome:
  • 09, 13

From Takeshi Ohama, Kochi University of Technology-Japan, September 2018

This strain is good for overexpression of various transgenes. This is probably due to the relieved transcriptional repression.


Kurniasih SD, Yamasaki T, Kong F, Okada S, Widyaningrum D, Ohama T (2016) UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems. Plant Mol Biol. 92:629-641

From Takeshi Ohama, Kochi University of Technology-Japan, September 2018

This strain is good for overexpression of various transgenes. This is probably due to the relieved transcriptional repression.


Kurniasih SD, Yamasaki T, Kong F, Okada S, Widyaningrum D, Ohama T (2016) UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems. Plant Mol Biol. 92:629-641

From Takeshi Ohama, Kochi University of Technology-Japan, September 2018

This strain is good for overexpression of various transgenes. This is probably due to the relieved transcriptional repression.


Kurniasih SD, Yamasaki T, Kong F, Okada S, Widyaningrum D, Ohama T (2016) UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems. Plant Mol Biol. 92:629-641