Efficient CRISPR-based method for targeted disruption of genes in Chlamydomonas
In May 2020, the Witman and Oda labs published a paper titled “TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii” (PMC7219734). The paper describes an easy, rapid, and cost-effective method for efficiently targeting specific genes for disruption in Chlamydomonas. The authors tested TIM on six different genes in two different cell-walled strains … Continued
Postdoc position available in the George Witman lab at UMass Medical School
An NIH-funded postdoctoral position currently is available in the Witman lab. We study cilia and flagella (terms here used interchangeably) to understand how they are assembled and function and why defects in specific ciliary proteins cause human diseases. Most of our research utilizes Chlamydomonas reinhardtii, which has major advantages for studies of flagella; we also … Continued