MoClo Toolkit


The entire collection of plasmids, distributed either as live cultures or frozen glycerol stocks in a 96-well format, will be priced at $400 for academic/non-profit users and $800 for commercial/industry. Individual plasmids from the kit can also be ordered as bacterial cultures on agar plugs for standard plasmid pricing. You may view the individual list of plasmids in this collection here:

From Crozet et al. (2018): “… we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.”

MoClo Kit Sequences

MoClo Kit Documents

Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086

We are sorry but we cannot ship the MoClo Toolkit to China because of long shipping and customs delays. Individual clones can still be shipped however.