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pCM0-TB_014
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: MvELS (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: A0A075FBG7
Brick function: functional diterpene synthase from Marrubium vulgare, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_016
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: SsSCS (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: K4HYB0
Brick function: functional diterpene synthase from Salvia sclarea, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_018
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: SsLPPS (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: K4HZA9
Brick function: functional diterpene synthase from Salvia sclarea, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_020
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: CfTPS1 (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: X5A4D6
Brick function: functional diterpene synthase from Coleus forskohlii, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_022
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: TwTPS14 (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: A0A0M5L832
Brick function: functional diterpene synthase from Tripterygium wilfordii, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_026
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: CfTPS3 (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: X5A2Z7
Brick function: functional diterpene synthase from Coleus forskohlii, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_039
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: SpDXS_K284N (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: A0A4V1ES21
Brick function: functional diterpene synthase from salvia pomifera, mutated K284N, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_044
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B5
Brick name: CrGGPPS (CDS_B5)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: A8JHU6
Brick function: functional geranylgeranyl pyrophosphate synthase from Chlamydomonas reinhardtii, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pAGM1301
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_052
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: HlDXR (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: H2DQ70
Brick function: functional 1-deoxy-d-xylulose 5-phosphate reductoisomerase from Haematococcus lacustris, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_101
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: CfTPS2 (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: X4ZWN5
Brick function: functional diterpene synthase from Coleus forskohlii, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_124
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: ScIDI (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: P15496
Brick function: functional isopentenyl diphosphate isomerase from Saccharomyces cerevisiae, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM2-L2Acceptor
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Vector: pAGM4673
Host strain: DH5a
Selection: Kanamycin (50 μg/ml)
Origin: synthetic construct
Insert: modified L2 vector, with flanking SspI sites, SspI-containing KanR and LacZa element
Function: modified L2 acceptor vector for L2-assembly, provides SspI sites flanking GOI expression positions and within KanR to linearize and inactivate bacterial resistance gene in a single step, Cred cassette was replaced by LacZa for blue/white screening after assembly, see Einhaus et al. 2022 for detailed description.
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pMBS807
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, March 2022
Host strain: TOP10
Selection in E. coli: kanamycin (50 μg/ ml)
Position L0 (Patron et al):
Brick name: L2-aadA-CRed (SpecR)
Brick type: Destination vector
Sequence reference:
Brick function: Destination vector with L1 aadA (SpecR) and CRed accepting L0 parts
Source of Brick: Niemeyer and Schroda 2022
Acceptor plasmid: pAGM4673
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Schroda M. New destination vectors facilitate Modular Cloning for Chlamydomonas. Curr Genet. 2022 Aug;68(3-4):531-536. doi: 10.1007/s00294-022-01239-x. Epub 2022 Apr 16. PMID: 35429260; PMCID: PMC9279246.
pMBS808
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, March 2022
Host strain: TOP10
Selection in E. coli: kanamycin (50 μg/ ml)
Position L0 (Patron et al):
Brick name: L2-AphVIII-CRed (ParoR)
Brick type: Destination vector
Sequence reference:
Brick function: Destination vector with L1 aphVIII (ParoR) and CRed accepting L0 parts
Source of Brick: Niemeyer and Schroda 2022
Acceptor plasmid: pAGM4673
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Schroda M. New destination vectors facilitate Modular Cloning for Chlamydomonas. Curr Genet. 2022 Aug;68(3-4):531-536. doi: 10.1007/s00294-022-01239-x. Epub 2022 Apr 16. PMID: 35429260; PMCID: PMC9279246.
pMBS809
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, March 2022
Host strain: TOP10
Selection in E. coli: kanamycin (50 μg/ ml)
Position L0 (Patron et al):
Brick name: L2-NAT-CRed (NTCR)
Brick type: Destination vector
Sequence reference:
Brick function: Destination vector with L1 nat1 (NTCR) and CRed accepting L0 parts
Source of Brick: Niemeyer and Schroda 2022
Acceptor plasmid: pAGM4673
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Schroda M. New destination vectors facilitate Modular Cloning for Chlamydomonas. Curr Genet. 2022 Aug;68(3-4):531-536. doi: 10.1007/s00294-022-01239-x. Epub 2022 Apr 16. PMID: 35429260; PMCID: PMC9279246.
pMBS810
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, March 2022
Host strain: TOP10
Selection in E. coli: kanamycin (50 μg/ ml)
Position L0 (Patron et al):
Brick name: L2-AphVII-CRed (HygroR)
Brick type: Destination vector
Sequence reference:
Brick function: Destination vector with L1 aphVII (HygroR) and CRed accepting L0 parts
Source of Brick: Niemeyer and Schroda 2022
Acceptor plasmid: pAGM4673
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: http://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Schroda M. New destination vectors facilitate Modular Cloning for Chlamydomonas. Curr Genet. 2022 Aug;68(3-4):531-536. doi: 10.1007/s00294-022-01239-x. Epub 2022 Apr 16. PMID: 35429260; PMCID: PMC9279246.