* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-64 er-u-1a mt+
$30.00
From Lawrence Bogorad to Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (erythromycin)
This is a good chloroplast marker for erythromycin resistance, and has been used in many crosses. It maps to the 3′ end of the 23S rRNA gene, distal to the intron, and is a C-> T base change in the 23S rRNA at the position equivalent to E. coli 2611.
Mets LJ and Bogorad L (1971) Mendelian and uniparental alterations in erythromycin binding by plastid ribosomes. Science 174:707-709
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292