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CC-73 ery3a mt-

Name: CC-73 ery3a mt-
SKU: CC-73
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University

Phenotype: antibiotic resistant (erythromycin)

The ery3a mutation was obtained in the Boynton/Gillham laboratory, induced by mutagenesis with nitrosoguanidine and shown to be equivalent to ery-2y in Surzycki and Gillham (1971) and papers by Chiang and colleagues.

This is a very useful strain for mapping on the right arm of linkage group I, easy to score as resistant to 100 micrograms/ml erythromycin on minimal medium.

We suspect the ERY3 locus corresponds to the PRPL34 gene, encoding chloroplast ribosomal protein L34.

Surzycki SJ and Gillham NW (1971) Organelle mutations and their expression in Chlamydomonas reinhardi. Proc. Natl Acad Sci USA 68:1301-1306

Davidson JN, Hanson MR, Bogorad L (1978) Erythromycin resistance and the chloroplast ribosome in Chlamydomonas reinhardi. Genetics 89:281-297

Locus:
ERY3
Chromosome:
1

CC-73 ery3a mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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