CC-86 nr-u-2-1 mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (kanamycin or neamine)
Product from a cross of wild type mt+ (N.W. Gillham’s strain, equivalent to CC-125) to nr-u-2-1 sr-u-2-60 mt-.
This is a useful chloroplast marker, mapping to the 3′ end of the chloroplast 16S ribosomal RNA. The mutation is an A>G change at nucleotide 1340. Although isolated initially as a neamine-resistant strain, it is also resistant to kanamycin, a more readily available antibiotic. CC-86 was used in many crosses in the Boynton-Gillham laboratory in the 1970s.
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292