CC-2255 dum1 (cob1 deletion) mt-
$30.00
From Rene Matagne, University of Liege, May 1988
Phenotype: dies in the dark
This is a terminal deletion mutation in mitochondrial DNA that eliminates the cob (cytochrome b) gene. It is an obligate photoautotroph, i.e. cannot grow on acetate medium in the dark. DNA analysis shows both monomer and dimer forms of the genome, and a heterogeneous size distribution.
CC-2654 is a subclone of this strain.
Matagne RF, Michel-Wolwertz MR, Munaut C, Duyckaerts C, Sluse F (1989) Induction and characterization of mitochondrial DNA mutants in Chlamydomonas reinhardtii. J Cell Biol 108:1221-1226
Randolph-Anderson BL, Boynton JE, Gillham NW, Harris EH, Johnson AM, Dorthu MP, Matagne RF (1993) Further characterization of the respiratory deficient dum-1 mutation of Chlamydomonas reinhardtii and its use as a recipient for mitochondrial transformation. Mol Gen Genet 236:235-244