CC-2419 cbn1-113 [= pg-113]
$30.00
From Alexander Chunayev, Leningrad State University, September 1989
Phenotype: somewhat chlorophyll deficient
The pg-113 mutant was isolated by M. Tellenbach in Arminio Boschetti’s laboratory, and subsequently determined in collaboration with Chunayev to be an allele at the CBN1 locus, now known to be equivalent to the CAO1 gene. It is somewhat chlorophyll deficient and should be maintained in dim light.
The following notes on pg-113 were sent by Armino Boschetti in 2001:
“The pg-113 mutant was obtained by M. Tellenbach by UV mutation of C. reinhardtii strain 11/32f Levine, mutant Arg2 mt+ (from Culture Collection of Algae and Protozoa, Cambridge UK) and selected for altered color [M. Tellenbach (1984), Herbizidresistenz und Pigmentdefizienz, Ph.D. thesis, University of Bern, Switzerland]. It was described in several papers, but the mutated gene or gene product is not yet identified. In collaboration with A.S. Chunayev it was shown that this mutant is allelic to his cbn-48-1 mutant, however, the composition of the thylakoid proteins seem to be somehow different. The mutant laks a stable chlorophyll complex II (electrophoresis of partially solubilized thylakoids) and lacks chlorophyll b, but has normal chlorophyll a content. The amount of neoxanthin is reduced. The LHCII proteins are present and are even phosphorylated and dephosphorylated as normal. ”
For more information on cbn1, please see CC-1354.
Michel H, Tellenbach M, Boschetti A (1983) A chlorophyll b-less mutant of Chlamydomonas reinhardii lacking in the light-harvesting chlorophyll complex but not in its apoproteins. Biochim Biophys Acta 725:417-424