* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-2454 nit1-305 cw15 mt-
$30.00
From P.A. Lefebvre, University of Minnesota, January 1990
Phenotype: wall deficient
The original nit1 mutation confers deficiency in both the diaphorase and terminal activities of nitrate reductase; nit1-305 affects only the diaphorase activity. This strain is transformable with the NIT1 plasmid. The cell wall deficient phenotype is not pronounced in this strain, however, and better transformation efficiencies will be obtained if autolysin is also used to remove the walls prior to introducing the DNA. This strain has the wild type allele at the NIT2 locus.
For more information on nit1-305, please see CC-2453.