CC-2471 pc1 mt-
$30.00
From Wei-Yeh Wang, University of Iowa, March 1990; original culture lost, replaced by Wang in 1994
This mutant cannot photoconvert protochlorophyllide. Although it is not light-sensitive, we maintain it in the dark to avoid selecting for reversion or suppression. This strain is green in the dark. Li and Timko determined that this is a deletion mutation in the gene encoding NADPH:protochlorophyllide oxidoreductase.
CC-3964 couples pc1 with the y7 mutation, which prevents dark enzymatic conversion of protochlorophyllide.
Ford C, Mitchell S, Wang W (1981) Protochlorophyllide photoconversion mutants of Chlamydomonas reinhardtii. Mol Gen Genet 184:460-464
Ford C, Mitchell S, Wang W (1983) Characterization of NADPH: Protochlorophyllide oxidoreductase in the y-7 and pc-1 y-7 mutants of Chlamydomonas reinhardtii. Mol Gen Genet 192:290-292
Li J, Timko MP (1996) The pc-1 phenotype of Chlamydomonas reinhardtii results from a deletion mutation in the nuclear gene for NADPH:protochlorophyllide oxidoreductase. Plant Mol Biol 30:15-37