CC-217 spr-u-sp-23 mt+
$30.00
From Ruth Sager, Hunter College, April 1974
Phenotype: antibiotic resistant (spectinomycin)
The spr-u-sp-23 mutant (originally sp23) was isolated by Sager and later shown by Harris et al. to have an A to C change at position 1123 in the chloroplast 16S rRNA. This mutation confers high level spectinomycin resistance under all growth conditions.
Like most of Sager’s strains, CC-217 grows on nitrate as sole N source (indicated by the NIT allele designation). This property is in contrast to most strains from the Ebersold/Levine background, which carry the nit1 and nit2 mutations.
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292