* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-2678 cw15 sr1 nit1-305 mt+
$30.00
From P.A. Lefebvre, University of Minnesota, September 1991
Phenotype: somewhat wall deficient; antibiotic resistant (streptomycin)
This strain is reported to work well as a recipient for transformation with the NIT1 (NIA1) gene. However, the cell-wall deficient phenotype is not as marked as in the original cw15 mutant. Better transformation efficiencies will be obtained if autolysin is also used to remove the walls prior to introducing the DNA. This strain has the wild type allele at the NIT2 locus.
For more information on nit1-305, please see CC-2453.