* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-2803 rbcL (disruption) sr-u-2-60 spr-u-1-6-2 mt+ [Newman 25B1]
$30.00
Scott Newman in Boynton-Gillham laboratory, Duke University, 1992
Phenotype: requires acetate; antibiotic resistant (spectinomycin, streptomycin)
This strain was obtained by co-transformation of CC-125 with P-183 (sr spr) and P-270, which is a BamHI-EcoRI fragment internal to the rbcL gene, with a Pst site replaced by insertion of a 0.48 kb fragment from the yeast plasmid YEp24.
Newman SM, Gillham NW, Harris EH, Johnson AM, Boynton JE (1991) Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii. Mol Gen Genet 230:65-74