CC-2831 psbA-NP (nonprocessed psbA) mt+
$30.00
Amnon Lers in Boynton-Gillham laboratory, Duke University
This is transformant NP-5, from transformation of CC-125 with DNA from plasmid P-400 (no longer in the collection) containing a site-directed mutation (psbA-NP) in the C-terminal extension of the psbA gene. The mutation changes a Ser (UCA) to stop (UAA) codon in the C-terminal extension of the D1 protein, thus causing the cells to produce a mature D1 protein that requires no further processing. Lers et al. found that these transformants are indisinguishable from wild type cells in terms of photosynthetic performance and growth rate under CO2- and light-saturated photoautotrophic growth conditions.
Lers A, Heifetz PB, Boynton JE, Gillham NW, Osmond CB (1992) The carboxyl-terminal extension of the D1 protein of photosystem II is not required for optimal photosynthetic performance under CO2- and light-saturated growth conditions. J Biol Chem 267:17494-17497