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CC-2926 iso1 mt-

Name: CC-2926 iso1 mt-
SKU: CC-2926
Availability: InStock

$30.00

From Ursula Goodenough, Washington University, July 1993

Phenotype: does not mate

The iso1 mutation is expressed only in minus gametes, but is not linked to the mating type locus. Cells in a culture of iso1 mutants differentiate either into minus or pseudo-plus cells, which can agglutinate with each another but do not fuse to form zygotes. This is an ARG7 insertional mutant obtained in CC-1861 arg7 mt- (now replaced in the collection by CC-3681), and is formally known as isolr:pARG7.8.

Campbell AM, Rayala HJ, Goodenough UW (1995) The iso1 gene of Chlamydomonas is involved in sex determination. Mol Biol Cell 6:87-95

Ferris PJ, Waffenschmidt S, Umen JG, Lin H, Lee JH, Ishida K, Kubo T, Lau J, Goodenough UW (2005) Plus and minus sexual agglutinins from Chlamydomonas reinhardtii. Plant Cell 17:597-615

Lee JH, Lin H, Joo S, Goodenough UW (2008) Early sexual origins of homeoprotein heterodimerization and evolution of the plant KNOX/BELL family. Cell 133:829-840

CC-2926 iso1 mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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