CC-266 sr-u-sm3 msr1 mt+
$30.00
From Ruth Sager, Hunter College, March 1975
Phenotype: antibiotic and inhibitor resistant (methionine sulfoximine, streptomycin)
This is the primary stock of sr-u-sm3 in the Chlamydomonas Resource Center collection. Other streptomycin resistance mutations, notably sr-u-2-60, have been more frequently used in crosses for chloroplast genetics, however. sr-u-sm3 is unique among chloroplast sr mutants examined in having an alteration in the extreme 5′ end of the 16S rRNA molecule (U -> G at nt 14, corresponding to E. coli 13). This is not the same as sr-u-sm3a, which is a mutation in a different region of the chloroplast 16S rRNA.
Like most of Sager’s strains, CC-266 grows on nitrate as sole N source (indicated by the nit+ or NIT allele designation). This property is in contrast to most strains from the Ebersold/Levine background, which carry the nit1 and nit2 mutations.
Sager R and Ramanis Z (1965) Recombination of nonchromosomal genes in Chlamydomonas. Proc Natl Acad Sci USA 53:1053-1061
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292