CC-270 sr-u-sm2 msr1 act1 mt+
$30.00
From Ruth Sager, Hunter College, March 1975
Phenotype: antibiotic and inhibitor resistant (cycloheximide, methionine sulfoximine, streptomycin)
This is one of the strains reportedly used by Sager in her series of papers on mapping chloroplast genes by pedigree and cosegregation analysis. Although labeled as sensitive to actidione (cycloheximide) and methionine sulfoximine when received, phenotypic tests at Duke showed that it is resistant to both compounds. The cycloheximide resistance marker was shown to be act1 in a cross to ac12 and pf12.
Like most of Sager’s strains, CC-270 grows on nitrate as sole N source (indicated by the nit+ or NIT allele designation). This property is in contrast to most strains from the Ebersold/Levine background, which carry the nit1 and nit2 mutations.
Please see CC-215 for more information on the sr-u-sm2 mutation.