* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-3045 imp3 arg2 mt-
$30.00
From Patrick Ferris, Goodenough lab, Washington University, February 1995
Phenotype: does not mate; requires arginine
Cells with the imp3 mutation agglutinate strongly with partners of opposite mating type, but the initial adhesion is lost, cell walls are not shed, and gamete fusion is rare. Saito et al. have shown this mutant to be defective in production of cAMP in response to flagellar adhesion.
Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186
Saito T, Small L, Goodenough UW (1993) Activation of adenylyl cyclase in Chlamydomonas reinhardtii by adhesion and by heat. J Cell Biol 122:137-147