CC-3272 gag1 (sag2) mt+

$30.00

From Olivier Vallon, Institut de Biologie Physico-Chimique, November 1995

Phenotype: does not mate

The gag1 mutation is an allele at the SAG2 locus. Mutants at this locus do not agglutinate with cells of either mating type, but can be mated as mt+ by artificial agglutination with antibodies.

Notes from Olivier Vallon, September 1999: “The sterility phenotype is sex-limited (expressed only in mt+ cells) but the gene is unlinked to the mating type locus. The primary defect is in O-glycosylation: at 32 deg C, in both mating types. The major O-glycoproteins show increased electrophoretic mobility and altered antigenicity. Cells form clumps on plates and palmelloids in liquid culture. These defects are not observed when cells are grown at 18 deg C. However, the sex-limited sterility phenotype is expressed regardless of the growth temperature.”

This strain is the one shown in Figure 1, lane 1, of the following paper:

Vallon O, Wollman FA (1995) Mutations Affecting O-Glycosylation in Chlamydomonas reinhardtii Cause Delayed Cell Wall Degradation and Sex-Limited Sterility. Plant Physiol 108:703-712