CC-3319 oda4-V43 mt+
$30.00
From George Witman, Worcester Foundation for Experimental Biology, January 1996
Phenotype: impaired motility
This mutant was generated by insertional mutagenesis of a nit1 agg1 mt+ parental strain, transformed with the plasmid pGP505 containing the wild-type NIT1 allele (see Pazour et al. 1995). NIT+ strains having a slow-swimming, jerky-swimming phenotype were selected. (This phenotype is typical of mutants lacking the outer dynein arms).
In this strain, the beta dynein heavy chain (ODA4) is deleted (see Figure 8 of Wilkerson et al. 1995). This deletion includes both the 3′ and 5′ ends and thus is presumed to be complete. The genome contains pUC119 but it is not known if this segregates with the deletion.
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440
Wilkerson CG, King SM, Koutoulis A, Pazour GJ, Witman GB (1995) The 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly. J Cell Biol 12:169-178