CC-6206 Ci_pAH04mCherry

$30.00

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Chlamydomonas incerta CC-3871, transformed with the vector pAH04mCherry. This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and expresses the fluorescent protein mCherry, which is directed to the cytoplasm.

The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source.

Dataset info: https://doi.org/10.5281/zenodo.14020362


Kang, K., Do Espirito Santo, É., Diaz, C. J., Oliver, A., Saxton, L., May, L., Mayfield, S., & Dutra Molino, J. V. (2024). Establishing the green algae Chlamydomonas incerta as a platform for recombinant protein production. https://doi.org/10.1101/2024.10.25.618925

Molino JV, de Carvalho JC, Mayfield SP. Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS One. 2018 Feb 6;13(2):e0192433.