* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-6255 NIES-2464; SpecR mt+

Name: CC-6255 NIES-2464; SpecR mt+
SKU: CC-6255
Availability: InStock

$30.00

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Spectinomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

CC-6255 NIES-2464; SpecR mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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