* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pAPN_FMG1A-ko1-AadA
$30.00
From Adrian Nievergelt, Max-Planck-Institute of Molecular Plant Physiology (MPI-MP), Potsdam, Germany, July 2025
Selection in E. coli: Ampicillin
Host strain: E. coli: DH5α
Insert: Homology arms for insertional ko of FMG1A
Vector: pAPN_crAadA
Description: Donor plasmid used to make CC-6222 with guide GGTCTCGCTGACCAGCACGGCGG. Prepared for transformation by digestion with BspQI.
Hoepfner LM, Nievergelt AP, Matrino F, Scholz M, Foster HE, Rodenfels J, von Appen A, Hippler M, Pigino G. Unwrapping the Ciliary Coat: High-Resolution Structure and Function of the Ciliary Glycocalyx. Adv Sci (Weinh). 2025 Apr;12(16):e2413355. doi: 10.1002/advs.202413355. Epub 2025 Mar 5. PMID: 40041987; PMCID: PMC12021028.