CC-435 ac-u-b-1-10 spr-u-1-27-3 mt+
$30.00
Hurley Shepherd in Boynton-Gillham laboratory, Duke University
Phenotype: requires acetate; antibiotic resistant (spectinomycin)
From a cross of the original ac-u-b-1-10 mutant (CC-366, no longer in the collection) to an spr-u-1-27-3 strain derived from the precursor of CC-104.
The ac-u-b-1-10 mutant is deficient in ATP synthase activity, but the mutational lesion has not been identified. Chloroplast DNA appears normal when digested with BamHI or EcoRI.
The spr-u-1-27-3 mutation confers spectinomycin resistance. See CC-104 for additional information.
Shepherd HS, Boynton JE, Gillham NW (1979) Mutations in nine chloroplast loci of
Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci USA 76:1353-1357
Myers AM, Grant DM, Rabert DK, Harris EH, Boynton JE, Gillham NW (1982) Mutants of
Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid 7:133-151