* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-3833 pmh1
$30.00
From Malcolm Campbell, Davidson College, September 2000
Phenotype: does not mate
This mutant was obtained in a screen for mutants impaired in mating or zygote formation, and was characterized as being unable to agglutinate with gametes of either mating type. This is an insertional mutation made by transformation with the ARG7 gene. The affected gene was identified as the PMH1 gene, which encodes a plasma membrane hydrogen ATPase.
Campbell AM, Coble AJ, Cohen LD, Ch'Ng TH, Russo KM, Long EM, Armbrust EV (2001) Identification and DNA sequence of a new H+-ATPase in the unicellular green alga Chlamydomonas reinhardtii (CHLOROPHYCEAE). J. Phycol 37:536-642