CC-438 ac-u-i-2-25 spr-u-1-27-3 mt+
$30.00
Hurley Shepherd in Boynton-Gillham laboratory, Duke University
Phenotype: requires acetate; antibiotic resistant (spectinomycin)
The ac-u-i-2-25 mutant is deficient in chloroplast ATP synthase activity. The original mutant strain studied by Shepherd also lacked chlorophyll protein complex CP I and was originally thought to have a primary defect in this protein. Subsequent analysis by Bennoun (see Woessner et al., 1984) showed that this mutant has a leaky ATP synthase-deficient phenotype in dim light.
This information was published in a footnote to a table in Woessner et al. (1984); however this mutant was not included in the complementation/recombination matrix in that paper.
This mutation is closely linked genetically to Bennoun’s CP I deficient mutant C1.
See CC-104 for additional information on the spr-u-1-27-3 mutant.
Shepherd HS, Boynton JE, Gillham NW (1979) Mutations in nine chloroplast loci of Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci USA 76:1353-1357
Woessner JP, Masson A, Harris EH, Bennoun P, Gillham NW, Boynton JE (1984) Molecular and genetic analysis of the chloroplast ATPase of chlamydomonas. Plant Mol Biol 3:177-190