CC-3841 gli1 [Kozminski KKT1B3]

$30.00

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

The gli mutants were generated by Keith Kozminski using insertional mutagenesis, and are described in his Ph.D. thesis. The background strain was Kozminski’s KK30A3, a product from a cross between CC-1024 pf1 nit1 nit2 mt+ and KK25E5 nit1-305 mt- (which in turn was derived from a cross of a nit1305 mt+ strain to CC-2453 nit1305 mt-). Non-gliding mutants were selected by their compact colony morphology as described by Lewin (Experientia 38, 348-349, 1982).

The mutants were characterized by ability to adhere to a coverslip, to bind polystyrene beads to the flagellar membrane, and to translocate beads along the length of the flagellum.

Class I mutants are capable of all three of these activities.
Class II mutants adhere to coverslips but do not bind or translocate beads.
Class III mutants bind and translocate beads but do not adhere to coverslips.
Class IV mutants are deficient in all three activities.
Class V mutants adhere to coverslips, but only by their flagellar tips. They also bind and translocate beads.

Kozminksi concluded that different glycoproteins are probably involved in coverslip adhesion vs. binding beads (shown by classes II and III, and speculated that the Class IV mutants my be deficient in the previously characterized 350 kD protein of the flagellar membrane. He postulated that the Class I and Class V mutants could have alterations in regulation of gliding or in the gliding motor.

This mutant (T1B3) is in Class I.